Recombinant
RabMAb

Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)

Overview

  • Product name
    Anti-NMDAR2B (phospho S1303) antibody [EP1858Y]
    See all NMDAR2B primary antibodies
  • Description
    Rabbit monoclonal [EP1858Y] to NMDAR2B (phospho S1303)
  • Host species
    Rabbit
  • Specificity
    ab81271 detects NMDAR2B when phosphorylated at serine 1303
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding serine 1303 of human NMDAR2B.

  • Positive control
    • WB: Human, mouse and rat brain tissue lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab81271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 166 kDa.
ICC 1/100.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
    • Tissue specificity
      Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
    • Sequence similarities
      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
    • Cellular localization
      Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AW490526 antibody
      • EIEE27 antibody
      • Glutamate [NMDA] receptor subunit epsilon 2 antibody
      • Glutamate [NMDA] receptor subunit epsilon-2 antibody
      • Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
      • Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
      • Glutamate receptor ionotropic NMDA2B antibody
      • Glutamate receptor subunit epsilon 2 antibody
      • Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
      • GRIN 2B antibody
      • GRIN2B antibody
      • hNR 3 antibody
      • hNR3 antibody
      • MGC142178 antibody
      • MGC142180 antibody
      • MRD6 antibody
      • N methyl D asparate receptor channel subunit epsilon 2 antibody
      • N methyl D aspartate receptor subtype 2B antibody
      • N methyl D aspartate receptor subunit 2B antibody
      • N methyl D aspartate receptor subunit 3 antibody
      • N-methyl D-aspartate receptor subtype 2B antibody
      • N-methyl-D-aspartate receptor subunit 3 antibody
      • NMDA NR2B antibody
      • NMDA R2B antibody
      • NMDAR2B antibody
      • NMDE2 antibody
      • NMDE2_HUMAN antibody
      • NME2 antibody
      • NR2B antibody
      • NR3 antibody
      see all

    Images

    • All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution

      Lane 1 : Rat brain tissue lysate
      Lane 2 : Rat brain tissue lysate. The membrane was incubated with alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 166 kDa
      Observed band size: 180 kDa (why is the actual band size different from the predicted?)



      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution

      Lane 1 : Human brain tissue lysate
      Lane 2 : Human brain tissue lysate. The membrane was incubated with alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 166 kDa
      Observed band size: 180 kDa (why is the actual band size different from the predicted?)



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot blot analysis of NMDAR2B (phospho S1303) phospho peptide (Lane 1) and NMDAR2B non-phospho peptide (Lane 2) labelling NMDAR2B (phospho S1303) with ab81271 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution

      Lane 1 : Mouse brain lysates (untreated)
      Lane 2 : Mouse brain lysates (treated with AP)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP labelled IgG at 1/2000 dilution

      Predicted band size: 166 kDa
      Observed band size: 180 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Castany S  et al. Critical role of sigma-1 receptors in central neuropathic pain-related behaviours after mild spinal cord injury in mice. Sci Rep 8:3873 (2018). Read more (PubMed: 29497125) »
    • Wang Y  et al. Propofol-induced downregulation of NR2B membrane translocation in hippocampus and spatial memory deficits of neonatal mice. Brain Behav 7:e00734 (2017). Read more (PubMed: 28729940) »

    See all 7 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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