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Product name Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] See all NMDAR2B primary antibodies
Rabbit monoclonal [EP1858Y] to NMDAR2B (phospho S1303)
Specificity ab81271 detects NMDAR2B when phosphorylated at serine 1303
Tested applications Suitable for:
WB, ICC more details Unsuitable for:
IHC-P or IP
Mouse, Rat, Human
A phospho specific peptide corresponding to residues surrounding serine 1303 of human NMDAR2B.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-NMDAR2B (phospho S1303) antibody (HRP) [EP1858Y] ( ab196974)
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EP1858Y
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 166 kDa.
Application notes Is unsuitable for IHC-P or IP.
Function NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Tissue specificity Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
Sequence similarities Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
Cellular localization Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
Information by UniProt
Glutamate [NMDA] receptor subunit epsilon 2 antibody
Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] images
Western blot - NMDAR2B (phospho S1303) antibody [EPR1858Y] (ab81271)
All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution Lane 1 : mouse brain lysates (untreated) Lane 2 : mouse brain lysates (treated with AP) Lysates/proteins at 10 µg per lane. Secondary Goat anti-rabbit HRP labelled IgG at 1/2000 dilution Predicted band size : 166 kDa Observed band size : 180 kDa ( why is the actual band size different from the predicted?)
References for Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)
This product has been referenced in:
et al. Chronic nicotine exposure inhibits estrogen-mediated synaptic functions in hippocampus of female rats. Neurosci Lett 517:41-6 (2012).
Read more (PubMed: 22521583) »
et al. aCaMKII is differentially regulated in brain regions that exhibit differing sensitivities to ischemia and excitotoxicity. J Cereb Blood Flow Metab : (2012).
Read more (PubMed: 22929440) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"