Dot Blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)
Dot blot analysis of NMDAR2B (phospho S1303) phospho peptide (Lane 1) and NMDAR2B non-phospho peptide (Lane 2) labelling NMDAR2B (phospho S1303) with ab81271 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
Western blot - NMDAR2B (phospho S1303) antibody [EPR1858Y] (ab81271)
All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Mouse brain lysates (untreated) Lane 2 : Mouse brain lysates (treated with AP)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat anti-rabbit HRP labelled IgG at 1/2000 dilution
Wang Y et al. Propofol-induced downregulation of NR2B membrane translocation in hippocampus and spatial memory deficits of neonatal mice. Brain Behav7:e00734 (2017).
Read more (PubMed: 28729940) »
Wang XQ et al. Differential roles of hippocampal glutamatergic receptors in neuropathic anxiety-like behavior after partial sciatic nerve ligation in rats. BMC Neurosci16:14 (2015).
Read more (PubMed: 25884414) »