Overview

  • Product nameAnti-NMDAR2C antibody
    See all NMDAR2C primary antibodies
  • Description
    Rabbit polyclonal to NMDAR2C
  • Specificityab110 labels the 140 kDa band representing NR2C in Western blots of cerebellum. It also labels the 180 kDa NR2A and NR2B bands in hippocampus. Immunolabeling is blocked by preadsorption of the antibody with the immunogen.
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: IHC-Fr
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein corresponding to Rat NMDAR2C aa 25-130 (N terminal).
    Database link: Q14957

  • General notes

    ab110 can be used to demonstrate that the NMDAR2C is highly enriched in the cerebellum.

Properties

Applications

Our Abpromise guarantee covers the use of ab110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Predicted molecular weight: 140 kDa.
IP Use at an assay dependent concentration.

3 µl will quantitatively immunoprecipitate all NMDAR2C in 200 µg of rat cerebellum.

  • Application notesIs unsuitable for IHC-Fr.
  • Target

    • FunctionNMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
    • Tissue specificityMainly expressed in brain with predominant expression is in the cerebellum, also present in the hippocampus, amygdala, caudate nucleus, corpus callosum, subthalamic nuclei and thalamus. Detected in the heart, skeletal muscle and pancreas.
    • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2C/GRIN2C subfamily.
    • Cellular localizationCell membrane. Cell junction, synapse, postsynaptic cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • GluN2C antibody
      • Glutamate [NMDA] receptor subunit epsilon-3 antibody
      • Glutamate Receptor Ionotropic N Methyl D Aspartate 2C antibody
      • Glutamate receptor ionotropic, NMDA 2C antibody
      • Glutamate receptor, ionotropic, N methyl D aspartate 2C antibody
      • Grin2c antibody
      • N Methly D Aspartate Receptor Channel Subunit Epsilon 3 antibody
      • N Methyl D Aspartate Receptor Subtype 2C antibody
      • N methyl D aspartate receptor subunit 2C antibody
      • N-methyl D-aspartate receptor subtype 2C antibody
      • NMDAR2C antibody
      • NMDE3_HUMAN antibody
      • NR2C antibody
      • OTTHUMP00000041930 antibody
      see all

    Anti-NMDAR2C antibody images

    • Anti-NMDAR2C antibody (ab110) at 1/1000 dilution + Rat cerebellar lysate

      Predicted band size : 140 kDa
      Observed band size : 140 kDa

      Specific staining of the ~140k R2C subunit of the receptor.

    References for Anti-NMDAR2C antibody (ab110)

    This product has been referenced in:
    • Buret L & van den Buuse M Corticosterone treatment during adolescence induces down-regulation of reelin and NMDA receptor subunit GLUN2C expression only in male mice: implications for schizophrenia. Int J Neuropsychopharmacol 17:1221-32 (2014). WB ; Mouse . Read more (PubMed: 24556017) »
    • Pristerà A  et al. Impact of N-tau on adult hippocampal neurogenesis, anxiety, and memory. Neurobiol Aging 34:2551-63 (2013). Read more (PubMed: 23769395) »

    See all 6 Publications for this product

    Product Wall

    I have received confirmation from the laboratory that this Anti-NMDAR2C antibody has not been tested in IHC on paraffin-embedded tissue. It is possible it will work but I expect it will require heat-mediated antigen retrieval and tests of various antib...

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    Thank you for your enquiry. I am sorry that Ab110 did not work properly for you. Since you used it in a tested application with a species this antibody is known to cross-react with, this should have worked. If you would like to share further d...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"