Recombinant
RabMAb

Anti-NMDAR2C antibody [EPR19094] (ab182277)

Overview

  • Product name
    Anti-NMDAR2C antibody [EPR19094]
    See all NMDAR2C primary antibodies
  • Description
    Rabbit monoclonal [EPR19094] to NMDAR2C
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Mouse NMDAR2C aa 300-500. The exact sequence is proprietary.
    Database link: Q01098

  • Positive control
    • WB: Mouse NMDAR2C fragment recombinant protein; Mouse and rat cerebellum lysates; Human fetal brain lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR19094
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab182277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 135 kDa).

Target

  • Function
    NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
  • Tissue specificity
    Mainly expressed in brain with predominant expression is in the cerebellum, also present in the hippocampus, amygdala, caudate nucleus, corpus callosum, subthalamic nuclei and thalamus. Detected in the heart, skeletal muscle and pancreas.
  • Sequence similarities
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2C/GRIN2C subfamily.
  • Cellular localization
    Cell membrane. Cell junction, synapse, postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GluN2C antibody
    • Glutamate [NMDA] receptor subunit epsilon-3 antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate 2C antibody
    • Glutamate receptor ionotropic, NMDA 2C antibody
    • Glutamate receptor, ionotropic, N methyl D aspartate 2C antibody
    • Grin2c antibody
    • N Methly D Aspartate Receptor Channel Subunit Epsilon 3 antibody
    • N Methyl D Aspartate Receptor Subtype 2C antibody
    • N methyl D aspartate receptor subunit 2C antibody
    • N-methyl D-aspartate receptor subtype 2C antibody
    • NMDAR2C antibody
    • NMDE3_HUMAN antibody
    • NR2C antibody
    • OTTHUMP00000041930 antibody
    see all

Images

  • All lanes : Anti-NMDAR2C antibody [EPR19094] (ab182277) at 1/2000 dilution

    Lane 1 : Mouse NMDAR2C fragment recombinant protein
    Lane 2 : Mouse NMDAR2D fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 135 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane1: 1 second; Lane2: 3 minutes.

    Mouse NMDAR2C fragment recombinant protein contains aa300-499 with a His-Tag®. NMDAR2D fragment recombinant protein contains aa328-526 with a His-Tag®. These two recombinant proteins were made in-house.

  • All lanes : Anti-NMDAR2C antibody [EPR19094] (ab182277) at 1/1000 dilution

    Lane 1 : Rat cerebellum lysate
    Lane 2 : Mouse cerebellum lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : Rat liver lysate
    Lane 5 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile is consistent with what has been described in the literature (PMID: 8987814).

  • Anti-NMDAR2C antibody [EPR19094] (ab182277) at 1/1000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab182277 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Chicken Cell lysate - whole cell (Homogenized Cerebellum (Mouse/Chicken) Transfected)
Gel Running Conditions
Non-reduced Denaturing (4-20% Mini-PROTEAN­ TGX™ Precast Gels)
Loading amount
25 µg
Specification
Homogenized Cerebellum (Mouse/Chicken) Transfected
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mrs. Marthe Fjelldal

Verified customer

Submitted Feb 17 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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