Synthetic peptide conjugated to KLH, from within N terminal amino acids 1-100 of Human NME2. Please note that the immunogen used is 100% conserved in NME1 and it is likely that this antibody will alos recognise NME1 (not tested).
Mouse brain tissue lysate, Human breast carcinoma.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Use at an assay dependent concentration.
Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity.
Belongs to the NDK family.
Cytoplasm. Nucleus. Isoform 2 is mainly cytoplasmic and isoform 1 and isoform 2 are excluded from the nucleolus.
ab37627 at 1/50 dilution, staining NME2 in Human breast carcinoma by Immunohistochemistry, Formalin fixed Paraffin embedded tissue. ab37627 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.