The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).
RelevanceNME5 does not seem to have NDK kinase activity but confers protection from cell death by Bax and alters the cellular levels of several antioxidant enzymes including Gpx5. It may play a role in spermiogenesis by increasing the ability of late-stage spermatids to eliminate reactive oxygen species. NME5 is specifically expressed in testis germinal cells.