Overview

  • Product nameAnti-NMNAT2 antibody
    See all NMNAT2 primary antibodies
  • Description
    Rabbit polyclonal to NMNAT2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Chinese HamsterDoes not react with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Rat NMNAT2.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Rat Brain; Mouse Brain; Rat Cortex; Mouse Cortex; Rat Forebrain. This antibody gave a positive result in IF in the following Methanol fixed cell line: SKNSH

Properties

Applications

Our Abpromise guarantee covers the use of ab110040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionCatalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannnot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NAAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+).
  • Tissue specificityHighly expressed in brain, in particular in cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen. Also found in the heart, skeletal muscle, pancreas and islets of Langerhans.
  • PathwayCofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.
  • Sequence similaritiesBelongs to the eukaryotic NMN adenylyltransferase family.
  • Cellular localizationCytoplasm. Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1orf15 antibody
    • KIAA0479 antibody
    • MGC2756 antibody
    • NaMN adenylyltransferase 2 antibody
    • Nicotinamide mononucleotide adenylyltransferase 2 antibody
    • Nicotinamide nucleotide adenylyltransferase 2 antibody
    • Nicotinate-nucleotide adenylyltransferase 2 antibody
    • NMN adenylyltransferase 2 antibody
    • NMNA2_HUMAN antibody
    • NMNAT 2 antibody
    • Nmnat2 antibody
    • PNAT 2 antibody
    • PNAT2 antibody
    • Pyridine nucleotide adenylyltransferase 2 antibody
    see all

Anti-NMNAT2 antibody images

  • All lanes : Anti-NMNAT2 antibody (ab110040) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Rat Cortex Tissue Lysate
    Lane 4 : Mouse Cortex Tissue Lysate
    Lane 5 : Rat Forebrain Rat Tissue Lysate
    Lane 6 : Lung (Rat) Tissue Lysate
    Lane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24 kDa,68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ab110040 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab110040 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-NMNAT2 antibody (ab110040)

ab110040 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain from newborn pup)
Loading amount 40 µg
Specification brain from newborn pup
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Jon Gilley

Verified customer

Submitted Jan 10 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"