Anti-NMNAT3 antibody (ab71904)
Key features and details
- Mouse polyclonal to NMNAT3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NMNAT3 antibody
See all NMNAT3 primary antibodies -
Description
Mouse polyclonal to NMNAT3 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human NMNAT3. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- NMNAT3 transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab71904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 28 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 28 kDa. |
Target
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Function
Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD (+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Protects against axonal degeneration following injury. -
Tissue specificity
Expressed in lung and spleen with lower levels in placenta and kidney. -
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1. -
Sequence similarities
Belongs to the eukaryotic NMN adenylyltransferase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 349565 Human
- Omim: 608702 Human
- SwissProt: Q96T66 Human
- Unigene: 208673 Human
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Alternative names
- NaMN adenylyltransferase 3 antibody
- Nicotinamide mononucleotide adenylyltransferase 3 antibody
- Nicotinamide nucleotide adenylyltransferase 3 antibody
see all
Images
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All lanes : Anti-NMNAT3 antibody (ab71904) at 1/500 dilution
Lane 1 : NMNAT3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab71904 has been referenced in 1 publication.
- Zhu Y et al. Human Nmnat1 Promotes Autophagic Clearance of Amyloid Plaques in a Drosophila Model of Alzheimer's Disease. Front Aging Neurosci 14:852972 (2022). PubMed: 35401143