Overview

  • Product nameAnti-NMRAL1 antibody
    See all NMRAL1 primary antibodies
  • Description
    Mouse polyclonal to NMRAL1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human NMRAL1 protein (NP_065728).

  • Positive control
    • NMRAL1 transfected 293T lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

Target

  • FunctionRedox sensor protein. Undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP(+) levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. The homodimer binds one molecule of NADPH. Has higher affinity for NADPH than for NADP(+). Binding to NADPH is necessary to form a stable dimer.
  • Sequence similaritiesBelongs to the NmrA-type oxidoreductase family.
  • Cellular localizationCytoplasm. Cytoplasm > perinuclear region. Nucleus. Under normal redox growth conditions localizes in the cytoplasm and perinuclear region. Nuclear localization is promoted by increased intracellular nitric oxide and reduced NADPH/NADP(+) ratios.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ25918 antibody
    • HSCARG antibody
    • NmrA like family domain containing 1 antibody
    • NmrA like family domain containing protein 1 antibody
    • NmrA-like family domain-containing protein 1 antibody
    • NMRAL1 antibody
    • NMRL1_HUMAN antibody
    • SDR48A1 antibody
    see all

Anti-NMRAL1 antibody images

  • All lanes : Anti-NMRAL1 antibody (ab69203) at 1/500 dilution

    Lane 1 : NMRAL1 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

References for Anti-NMRAL1 antibody (ab69203)

ab69203 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"