Overview

  • Product nameAnti-NMRAL1 antibody
    See all NMRAL1 primary antibodies
  • Description
    Rabbit polyclonal to NMRAL1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (TCRHTAEEYA ALLTKHTRKV VHDAKMTPED YEKLGFPGAR DLANMFRFYA) of human NMRAL1 (NP_065728).

  • Positive control
    • Hela cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionRedox sensor protein. Undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP(+) levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. The homodimer binds one molecule of NADPH. Has higher affinity for NADPH than for NADP(+). Binding to NADPH is necessary to form a stable dimer.
  • Sequence similaritiesBelongs to the NmrA-type oxidoreductase family.
  • Cellular localizationCytoplasm. Cytoplasm > perinuclear region. Nucleus. Under normal redox growth conditions localizes in the cytoplasm and perinuclear region. Nuclear localization is promoted by increased intracellular nitric oxide and reduced NADPH/NADP(+) ratios.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ25918 antibody
    • HSCARG antibody
    • NmrA like family domain containing 1 antibody
    • NmrA like family domain containing protein 1 antibody
    • NmrA-like family domain-containing protein 1 antibody
    • NMRAL1 antibody
    • NMRL1_HUMAN antibody
    • SDR48A1 antibody
    see all

Anti-NMRAL1 antibody images

  • Anti-NMRAL1 antibody (ab86565) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG should at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 33 kDa

References for Anti-NMRAL1 antibody (ab86565)

ab86565 has not yet been referenced specifically in any publications.

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