Anti-nmt55 / p54nrb antibody (ab50952)


  • Product nameAnti-nmt55 / p54nrb antibody
    See all nmt55 / p54nrb primary antibodies
  • Description
    Rabbit polyclonal to nmt55 / p54nrb
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within synthetic peptide: GTLGLTPPTT ERFGQAATME GIGAIGGTPP AFNRAAPGAE FAPNKRRRY, corresponding to C terminal amino acids 423-471 of Human nmt55/ p54nrb

  • Positive control
    • HepG2 cell lysate; paraffin-embedded human liver tissue, paraffin-embedded human kidney tissue


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab50952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 8 µg/ml.


  • FunctionDNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site (By similarity). Involved in pre-mRNA splicing, probably as an heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. Nono is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
  • Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.
  • Involvement in diseaseNote=A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3.
  • Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    The N-terminus is blocked.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 52 kDa subunit antibody
    • 54 kDa nuclear RNA and DNA binding protein antibody
    • 54 kDa nuclear RNA- and DNA-binding protein antibody
    • 55 kDa nuclear protein antibody
    • DNA binding p52/p100 complex 52 kDa subunit antibody
    • DNA-binding p52/p100 complex antibody
    • NMT 55 antibody
    • NMT55 antibody
    • Non Pou domain containing octamer (ATGCAAAT) binding protein antibody
    • Non POU domain containing octamer binding antibody
    • Non POU domain containing octamer binding protein antibody
    • Non-POU domain-containing octamer-binding protein antibody
    • Nono antibody
    • NonO protein antibody
    • NONO_HUMAN antibody
    • NRB 54 antibody
    • NRB antibody
    • NRB54 antibody
    • Nuclear RNA binding protein 54kD antibody
    • P54 antibody
    • p54(nrb) antibody
    • p54nrb antibody
    • PPP1R114 antibody
    • Protein phosphatase 1 regulatory subunit 114 antibody
    see all

Anti-nmt55 / p54nrb antibody images

  • Anti-nmt55 / p54nrb antibody (ab50952) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • Tissue: paraffin-embedded human Liver
    Cells with positive label: Hepatocytes (indicated with arrows)
    Antibody concentration: 4 - 8 µg/ml
    Magnification: 400X
  • Tissue: paraffin-embedded human Kidney
    Cells with positive label: Epithelial cells of renal tubule (indicated with arrows)
    Antibody concentration: 4 - 8 µg/ml
    Magnification: 400X
  • ab50952 staining nmt55 / p54nrb in Hela cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-nmt55 / p54nrb antibody (ab50952)

ab50952 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human brain motor cortex)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Specification Human brain motor cortex
Blocking step (agent) for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative Formalin

Guinevere Lourenco

Verified customer

Submitted Nov 20 2015