Overview

  • Product nameAnti-NNF1R antibody
    See all NNF1R primary antibodies
  • Description
    Rabbit polyclonal to NNF1R
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Goat, Horse, Guinea pig, Pig
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Human stomach tissue. Transfected 293 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab56220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

Anti-NNF1R antibody images

  • Anti-NNF1R antibody (ab56220) at 1 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling NNF1R with ab56220 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of fundic gland. Magnification: 400X.

References for Anti-NNF1R antibody (ab56220)

ab56220 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"