Anti-NNMT antibody [OTI3D8] (ab119758)
Key features and details
- Mouse monoclonal [OTI3D8] to NNMT
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-NNMT antibody [OTI3D8]
See all NNMT primary antibodies -
Description
Mouse monoclonal [OTI3D8] to NNMT -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
Recombinant full length Human NNMT, produced in HEK293T cells (NP_006160).
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Positive control
- WB: HeLa, A549, HepG2 and SVT2 cell lysates; HEK293T cell lysate transfected with pCMV6-ENTRY NNMT cDNA. IHC-P: Human Liver, Pancreas, Thyroid carcinoma, Endometrium, Endometrium adenocarcinoma and Prostate tissues. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY NNMT. Flow Cyt (Intra): Jurkat cells.
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General notes
The clone number has been updated from 3D8 to OTI3D8, both clone numbers name the same clone.
This product was changed from ascites to tissue culture supernatant on 29th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab119758 is purified from TCS by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI3D8 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 30 kDa.
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IHC-P |
1/150.
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ICC/IF |
1/100.
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Flow Cyt (Intra) |
1/100.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 30 kDa. |
IHC-P
1/150. |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/100. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. -
Tissue specificity
Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas. -
Sequence similarities
Belongs to the NNMT/PNMT/TEMT family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4837 Human
- Entrez Gene: 18113 Mouse
- Omim: 600008 Human
- SwissProt: P40261 Human
- SwissProt: O55239 Mouse
- Unigene: 503911 Human
- Unigene: 623245 Human
- Unigene: 458542 Mouse
see all -
Alternative names
- EC 2.1.1.1 antibody
- Nicotinamide N methyltransferase antibody
- Nicotinamide N-methyltransferase antibody
see all
Images
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All lanes : Anti-NNMT antibody [OTI3D8] (ab119758) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : NNMT knockout HeLa cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDaLanes 1-3: Merged signal (red and green). Green - ab119758 observed at 30 kDa. Red - loading control ab181602 observed at 36 kDa.
ab119758 Anti-NNMT antibody [OTI3D8] was shown to specifically react with NNMT in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265700 (knockout cell lysate ab258537) was used. Wild-type and NNMT knockout samples were subjected to SDS-PAGE. ab119758 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated at room temperature for 2.5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human liver tissue by Immunohistochemistry.
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All lanes : Anti-NNMT antibody [OTI3D8] (ab119758) at 1/500 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY NNMT cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 30 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200641) -
All lanes : Anti-NNMT antibody [OTI3D8] (ab119758) at 1/500 dilution
Lane 1 : HepG2 cell extracts
Lane 2 : HeLa cell extracts
Lane 3 : SVT2 cell extracts
Lane 4 : A549 cell extracts
Lane 5 : COS7 cell extracts
Lane 6 : Jurkat cell extracts
Lane 7 : MDCK cell extracts
Lane 8 : PC12 cell extracts
Lane 9 : MCF7 cell extracts
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200641) -
ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
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ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
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ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
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ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
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ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human prostate tissue by Immunohistochemistry.
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ab119758, at 1/100 dilution staining NNMT, in COS7 cells transiently transfected by pCMV6-ENTRY NNMT, by Immunofluorescence
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ab119758 at 1/100 dilution staining NNMT in Jurkat cells by Flow Cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab119758 has been referenced in 12 publications.
- Zhang W et al. Identification of Biological Functions and Prognostic Value of NNMT in Oral Squamous Cell Carcinoma. Biomolecules 12:N/A (2022). PubMed: 36291696
- Zhen X et al. Nicotinamide Supplementation Attenuates Renal Interstitial Fibrosis via Boosting the Activity of Sirtuins. Kidney Dis (Basel) 7:186-199 (2021). PubMed: 34179114
- Yu J et al. Long non-coding RNA XIST promotes osteoporosis by inhibiting the differentiation of bone marrow mesenchymal stem cell by sponging miR-29b-3p that suppresses nicotinamide N-methyltransferase. Bioengineered 12:6057-6069 (2021). PubMed: 34486487
- Li Q et al. Heterogeneity of cell composition and origin identified by single-cell transcriptomics in renal cysts of patients with autosomal dominant polycystic kidney disease. Theranostics 11:10064-10073 (2021). PubMed: 34815804
- Cao Q et al. Long Non-coding RNA ST8SIA6-AS1 Promotes Lung Adenocarcinoma Progression Through Sponging miR-125a-3p. Front Genet 11:597795 (2020). PubMed: 33363573