Overview

  • Product nameAnti-NNMT antibody
    See all NNMT primary antibodies
  • Description
    Rabbit polyclonal to NNMT
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC, corresponding to amino acids 1-50 of Human NNMT

  • Positive control
    • HepG2 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab58743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 30 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

Anti-NNMT antibody images

  • Anti-NNMT antibody (ab58743) at 1/2.5 dilution + HepG2 cell lysate at 10 µg with skim milk/PBS buffer at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1: 50,000 - 100,000

    Predicted band size : 30 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)

References for Anti-NNMT antibody (ab58743)

This product has been referenced in:
  • Fedorowicz A  et al. Activation of the nicotinamide N-methyltransferase (NNMT)-1-methylnicotinamide (MNA) pathway in pulmonary hypertension. Respir Res 17:108 (2016). IHC-Fr ; Rat . Read more (PubMed: 27581040) »
  • Ulanovskaya OA  et al. NNMT promotes epigenetic remodeling in cancer by creating a metabolic methylation sink. Nat Chem Biol 9:300-6 (2013). Human . Read more (PubMed: 23455543) »

See all 2 Publications for this product

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