Overview

  • Product name
    Anti-NNMT antibody [OTI3D8]
    See all NNMT primary antibodies
  • Description
    Mouse monoclonal [OTI3D8] to NNMT
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length Human NNMT, produced in HEK293T cells (NP_006160).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY NNMT cDNA; HepG2 and SVT2 cell extracts; Human Liver, Pancreas, Thyroid carcinoma, Endometrium, Endometrium adenocarcinoma and Prostate tissues; COS7 cells transiently transfected by pCMV6-ENTRY NNMT; Jurkat cells.
  • General notes

    The clone number has been updated from 3D8 to OTI3D8, both clone numbers name the same clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab119758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 30 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • Function
    Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
  • Tissue specificity
    Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.
  • Sequence similarities
    Belongs to the NNMT/PNMT/TEMT family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.1.1.1 antibody
    • Nicotinamide N methyltransferase antibody
    • Nicotinamide N-methyltransferase antibody
    • NNMT antibody
    • NNMT_HUMAN antibody
    see all

Images

  • All lanes : Anti-NNMT antibody [OTI3D8] (ab119758) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY NNMT cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 30 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200641)
  • All lanes : Anti-NNMT antibody [OTI3D8] (ab119758) at 1/500 dilution

    Lane 1 : HepG2 cell extracts
    Lane 2 : HeLa cell extracts
    Lane 3 : SVT2 cell extracts
    Lane 4 : A549 cell extracts
    Lane 5 : COS7 cell extracts
    Lane 6 : Jurkat cell extracts
    Lane 7 : MDCK cell extracts
    Lane 8 : PC12 cell extracts
    Lane 9 : MCF7 cell extracts

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 30 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200641)
  • ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human liver tissue by Immunohistochemistry.
  • ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
  • ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
  • ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
  • ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • ab119758, at 1/100 dilution staining NNMT, in COS7 cells transiently transfected by pCMV6-ENTRY NNMT, by Immunofluorescence
  • ab119758 at 1/100 dilution staining NNMT in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

References

This product has been referenced in:
  • Mobley A  et al. Aurora Kinase A is a Biomarker for Bladder Cancer Detection and Contributes to its Aggressive Behavior. Sci Rep 7:40714 (2017). Read more (PubMed: 28102366) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U87 and U373 Human glioblastoma cell lines)
Loading amount
8000 cells
Specification
U87 and U373 Human glioblastoma cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Desire Ngoga

Verified customer

Submitted Jun 26 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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