Overview

  • Product name
    Anti-nNOS (neuronal) antibody
    See all nNOS (neuronal) primary antibodies
  • Description
    Goat polyclonal to nNOS (neuronal)
  • Tested applications
    Suitable for: IHC-FrFl, WB, IHC-FoFr, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human, Common marmoset
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide: ESKKDTDEVFSS, corresponding to amino acids 1423-1434 of Human NOS1.

  • Positive control
    • WB: mouse brain lysate IHC: mouse DRG following spinal nerve ligation (2 weeks)

Properties

Applications

Our Abpromise guarantee covers the use of ab1376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45, 160 kDa (predicted molecular weight: 161 kDa).Can be blocked with Human nNOS (neuronal) peptide (ab22863).

Lyse in RIPA buffer.

IHC-FoFr 1/300. Ovenight incubation at room temp.
IHC-P Use at an assay dependent concentration. PubMed: 16263087
IHC-Fr 1/1000. PubMed: 22589245

Target

  • Function
    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • Tissue specificity
    Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
  • Sequence similarities
    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.
  • Domain
    The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
  • Post-translational
    modifications
    Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • Cellular localization
    Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310005C01Rik antibody
    • BNOS antibody
    • Constitutive NOS antibody
    • EC 1.14.13.39 antibody
    • IHPS 1 antibody
    • IHPS1 antibody
    • N-NOS antibody
    • NC-NOS antibody
    • neuronal Nitric Oxide Synthase antibody
    • Neuronal NOS antibody
    • Nitric oxide synthase , neuronal, included antibody
    • Nitric oxide synthase 1 (neuronal) antibody
    • Nitric oxide synthase 1 antibody
    • Nitric oxide synthase, brain antibody
    • Nitric oxide synthase, penile neuronal, included antibody
    • NNOS antibody
    • NO antibody
    • NOS 1 antibody
    • NOS antibody
    • NOS type I antibody
    • NOS-I antibody
    • NOS1 antibody
    • NOS1_HUMAN antibody
    • Peptidyl-cysteine S-nitrosylase NOS1 antibody
    see all

Images

  • Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded marmoset cerebellum tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.

    See Abreview

  • ab1376 nNos antibody immunostaining nNos in dorsal root ganglion (DRG) neurons of an animal who had a spinal nerve ligation 2 weeks previous. [A] Image taken X10 objective, [B]Image taken with X20 objective; the staining is restricted to a subpopulation of neurons and is localized in the cytoplasm of these cells; Scale bar=20 microns. ab1376 was used at 1/300 overnight incubation at room temperature on 30 micron sections of PFA perfusion fixed DRG tissue (on slide). Staining visualised using anti goat FITC secondary.
  • Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded rat cerebral tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.

    See Abreview

  • IHC-Fr image of nNOS staining on marmoset caudate sections using ab1376 (1:1000). The tissue was fixed in paraformaldehyde and the sections were then permeabilized using Triton-X. The sections were them blocked using 2% BSA for 1 hour at 20°C. ab1376 was diluted 1:1000 and incubated with the sections for 18 hours at 20°C. The secondary antibody used was HRP conjugated Rabbit polyclonal to Goat IgG (undiluted)

    See Abreview

  • Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded mouse cerebral tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrival was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.

    See Abreview



  • Predicted band size : 161 kDa
    ab1376 (0.3 µg/ml) staining nNOS (neuronal) in human muscle whole tissue lysate (35 µg in RIPA). Incubation for 1 hour; detected by chemiluminescence.
  • Immunohistochemical analysis of foraldehyde-fixed paraffin-embedded dog cerebral tissue sections, labelling nNOS (neuronal) with ab1376 at a dilution of 1/2000 incubated at 21°C for 2 hours in TBS, azide & BSA diluent. Heat mediated antigen retrieval was with citric acid. Blocking was with 1% BSA at 21°C for 10 minutes. Secondary was a Horse anti-Goat polyclonal biotin conjugate at 1/300.

    See Abreview

References

This product has been referenced in:
  • Timpani CA  et al. Attempting to Compensate for Reduced Neuronal Nitric Oxide Synthase Protein with Nitrate Supplementation Cannot Overcome Metabolic Dysfunction but Rather Has Detrimental Effects in Dystrophin-Deficient mdx Muscle. Neurotherapeutics 14:429-446 (2017). Mouse . Read more (PubMed: 27921261) »
  • Leist M  et al. Two types of interneurons in the mouse lateral geniculate nucleus are characterized by different h-current density. Sci Rep 6:24904 (2016). IF . Read more (PubMed: 27121468) »

See all 20 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (stomach)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate PH 6,6
Permeabilization
Yes - Triton X100 0.5%
Specification
stomach
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Mr. Julien Chevalier

Verified customer

Submitted Aug 08 2016

Application
IHC - Wholemount
Sample
Pig Tissue (Intestinal myenteric plexus)
Specification
Intestinal myenteric plexus
Username

Mr. Julien Chevalier

Verified customer

Submitted Aug 08 2016

Application
IHC - Wholemount
Sample
Human Tissue (Intestinal myenteric plexus)
Specification
Intestinal myenteric plexus
Username

Abcam user community

Verified customer

Submitted Aug 08 2016

Application
IHC - Wholemount
Sample
Rat Tissue (Intestinal myenteric plexus)
Specification
Intestinal myenteric plexus
Username

Mr. Julien Chevalier

Verified customer

Submitted Aug 08 2016

Application
IHC - Wholemount
Sample
Mouse Tissue (Intestinal myenteric plexus)
Specification
Intestinal myenteric plexus
Username

Mr. Julien Chevalier

Verified customer

Submitted Aug 08 2016

Application
Western blot
Sample
Human Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 18 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 08 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Jugular-Nodose ganglia)
Permeabilization
No
Specification
Jugular-Nodose ganglia
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Mr. Duc Dung Le

Verified customer

Submitted Jun 08 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 21 2015

1-10 of 25 Abreviews or Q&A

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