Overview

  • Product name
    Anti-nNOS (neuronal) antibody
    See all nNOS (neuronal) primary antibodies
  • Description
    Rabbit polyclonal to nNOS (neuronal)
  • Specificity
    This antibody reacts with iNOS, bNOS and eNOS.
  • Tested applications
    Suitable for: IHC-FoFr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to Mouse nNOS (neuronal) (C terminal).

  • Positive control
    • Tested with HUVEC cells and placenta cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab15203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 20207227
IHC-P 1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 150 kDa.

Target

  • Function
    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • Tissue specificity
    Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
  • Sequence similarities
    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.
  • Domain
    The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
  • Post-translational
    modifications
    Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • Cellular localization
    Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310005C01Rik antibody
    • BNOS antibody
    • Constitutive NOS antibody
    • EC 1.14.13.39 antibody
    • IHPS 1 antibody
    • IHPS1 antibody
    • N-NOS antibody
    • NC-NOS antibody
    • neuronal Nitric Oxide Synthase antibody
    • Neuronal NOS antibody
    • Nitric oxide synthase , neuronal, included antibody
    • Nitric oxide synthase 1 (neuronal) antibody
    • Nitric oxide synthase 1 antibody
    • Nitric oxide synthase, brain antibody
    • Nitric oxide synthase, penile neuronal, included antibody
    • NNOS antibody
    • NO antibody
    • NOS 1 antibody
    • NOS antibody
    • NOS type I antibody
    • NOS-I antibody
    • NOS1 antibody
    • NOS1_HUMAN antibody
    • Peptidyl-cysteine S-nitrosylase NOS1 antibody
    see all

Anti-nNOS (neuronal) antibody images

  • ab15203 staining nNOS (neuronal) in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Sections were deparaffinized, rehydrated and submitted to antigen retrieval and blocked with 5% non-fat dry milk and 0.1% Triton X-100. Samples were incubated with primary antibody (1/200 in PBS containing 2% non-fat dry milk and 0.05% Trition X-00) overnight at 4°C. A Biotin-conjugated anti-rabbit IgG (1/200 in PBS containing 1% NGS for 1 hour at room temperature) was used as the secondary antibody. Left - wild type, Right - Cre+/RTN-1C+. Magnification: 20x.

  • Human placenta tissue section stained with ab15203 by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

References for Anti-nNOS (neuronal) antibody (ab15203)

This product has been referenced in:
  • Tuncer AA  et al. The Protective Effects of Alpha-Lipoic Acid and Coenzyme Q10 Combination on Ovarian Ischemia-Reperfusion Injury: An Experimental Study. Adv Med 2016:3415046 (2016). IHC . Read more (PubMed: 27597986) »
  • Thuy le TT  et al. Cytoglobin deficiency promotes liver cancer development from hepatosteatosis through activation of the oxidative stress pathway. Am J Pathol 185:1045-60 (2015). Read more (PubMed: 25665792) »

See all 6 Publications for this product

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Mid LDT coronal sections)
Specification
Mid LDT coronal sections
Permeabilization
Yes - Triton X-100
Fixative
Paraformaldehyde
Username

Jason Teem

Verified customer

Submitted Jan 06 2014

I think we discussed this question when you called this morning. But just to reiterate, yes you can use ab96884 as a secondary to detect ab76319. Please let me know if you have any other questions.

I am very pleased to hear you would like to accept our offer and test ab15203 in ICC/IF. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and include this code in the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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