Overview

  • Product nameAnti-nNOS (neuronal) antibody
    See all nNOS (neuronal) primary antibodies
  • Description
    Sheep polyclonal to nNOS (neuronal)
  • SpecificityReacts with nNOS. Cross-reactivity with iNOS or eNOS not known, but not expected.
  • Tested applicationsWB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Guinea pig
  • Immunogen

    Synthetic peptide:

    RSESIAFIEESKKDADEVFSS

    conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 1409-1429 of Rat NOS1 (nNOS).

  • Positive control
    • rat brain
  • General notesThis antibody is a high quality immunohistochemical reagent that has been used for studies in both the peripheral and central nervous system. It is also useful for double label studies when combined with rabbit or other primary antibodies.

Properties

Applications

Our Abpromise guarantee covers the use of ab6175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. It has been reported that the antibody can give mild background in some instances.
IHC-P 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr 1/100 - 1/2000.

Target

  • FunctionProduces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • Tissue specificityIsoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
  • Sequence similaritiesBelongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.
  • DomainThe PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
  • Post-translational
    modifications
    Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • Cellular localizationCell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310005C01Rik antibody
    • bNOS antibody
    • Constitutive NOS antibody
    • EC 1.14.13.39 antibody
    • IHPS 1 antibody
    • IHPS1 antibody
    • N NOS antibody
    • N-NOS antibody
    • NC NOS antibody
    • NC-NOS antibody
    • NCNOS antibody
    • neuronal Nitric Oxide Synthase antibody
    • Neuronal NOS antibody
    • Nitric oxide synthase , neuronal, included antibody
    • Nitric oxide synthase 1 (neuronal) antibody
    • Nitric oxide synthase 1 antibody
    • Nitric oxide synthase brain antibody
    • Nitric oxide synthase, brain antibody
    • Nitric oxide synthase, penile neuronal, included antibody
    • nNOS antibody
    • NO antibody
    • NOS 1 antibody
    • NOS antibody
    • NOS type I antibody
    • NOS-I antibody
    • NOS1 antibody
    • NOS1_HUMAN antibody
    • Peptidyl-cysteine S-nitrosylase NOS1 antibody
    see all

Anti-nNOS (neuronal) antibody images

  • Rat braistem staining for nNOS with ab6175 (1:2000) (perfusion fixed frozen sections).

References for Anti-nNOS (neuronal) antibody (ab6175)

This product has been referenced in:
  • Colucci M  et al. Intestinal dysmotility and enteric neurochemical changes in a Parkinson's disease rat model. Auton Neurosci 169:77-86 (2012). Read more (PubMed: 22608184) »
  • Buchwalow IB  et al. Nitric oxide synthase in muscular dystrophies: a re-evaluation. Acta Neuropathol (Berl) 111:579-88 (2006). Read more (PubMed: 16718354) »

See all 3 Publications for this product

Product Wall

Thank you for your reply.

As for ab72428, I will need to check with the lab regarding tested tissue,localization of staining and potential cross-reactivity with eNOS and iNOS. I will letyou know what I find out. Thank you for your patience. ...

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Thank you for contacting us.

We have 3 antibodies that were tested and are guaranteed (http://www.abcam.com/Abpromise) for IHC-P and rabbit samples: ab1376,ab6175 and ab72428. I would recommend trying one of thosethat were tested as they are...

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I'm sorry to hear you are experiencing problems with ab6175, I have received feedback from the laboratory that informed me that your protocol is very similar to ours and we would expect it to work, except the addition of tritonx100 in the antibody dilu...

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All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity).

A positive control tissue for this antibody is the thoracic spinal cord (of rat). Pre-ganglionic sympathetic neurons stain for NOS.

We cannot provide a definitive answer to this query. However, it is unlikely that our antibody to bNOS recognizes inducible NOS.

This antibody, ab6175, is known to react with bNOS in rabbits.

Yes the antibody does cross react with mouse.

This anti-NOS antibody has not, to our knowledge, been tested in paraffin sections, but we would be surprised if this antibody did not work in paraffin. It is an antibody that is well suited to immunohistochemistry and we have found it works well for ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"