The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 161 kDa.
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
FunctionProduces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Tissue specificityIsoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
Sequence similaritiesBelongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain. Contains 1 PDZ (DHR) domain.
DomainThe PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
Post-translational modificationsUbiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
Cellular localizationCell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
Nitric oxide synthase , neuronal, included antibody
Nitric oxide synthase 1 (neuronal) antibody
Nitric oxide synthase 1 antibody
Nitric oxide synthase, brain antibody
Nitric oxide synthase, penile neuronal, included antibody
NOS 1 antibody
NOS type I antibody
Peptidyl-cysteine S-nitrosylase NOS1 antibody
Anti-nNOS (neuronal) antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NOS antibody (ab95436)
ab95436 at 2µg/ml staining NOS in rat brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). A heat mediated antigen retrieval step was performed.
Western blot - NOS antibody (ab95436)
All lanes : Anti-nNOS (neuronal) antibody (ab95436) at 1 µg/ml
Lane 1 : Lysate prepared from rat brain tissue. Lane 2 : Lysate prepared from rat brain tissue. Lane 3 : Lysate prepared from rat medulla oblongata tissue. Lane 4 : Whole cell lysate prepared from MCF7 cells. Lane 5 : Whole cell lysate prepared from SMMC cells. Lane 6 : Whole cell lysate prepared from SW620 cells. Lane 7 : Whole cell lysate prepared from Colo320 cells.
Predicted band size : 161 kDa
References for Anti-nNOS (neuronal) antibody (ab95436)
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