Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583)

Overview

  • Product nameAnti-nNOS (neuronal) (phospho S1417) antibodySee all nNOS (neuronal) primary antibodies ...
  • Description
    Rabbit polyclonal to nNOS (neuronal) (phospho S1417)
  • Tested applicationsIHC-FoFr, WB, IHC-Fr, ICC, IP, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
    Predicted to work with: Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human nNOS (neuronal) aa 1411-1425 (phospho S1417).
    Sequence: CNRLRSE(pS)IAFIEESK
    Database link: P29475
    (Peptide available as ab41773)

Properties

Applications

Our Abpromise guarantee covers the use of ab5583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 20881244
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 160 kDa.Can be blocked with Human nNOS (neuronal) (phospho S1417) peptide (ab41773).
IHC-Fr 1/500.
ICC 1/10 - 1/100.
IP Use at an assay dependent concentration.
ICC/IF 1/10 - 1/100.

Target

  • FunctionProduces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • Tissue specificityIsoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
  • Sequence similaritiesBelongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.
  • DomainThe PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
  • Post-translational
    modifications
    Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • Cellular localizationCell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
  • Target information above from: UniProt accession P29475 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 2310005C01Rik antibody
    • bNOS antibody
    • Constitutive NOS antibody
    • EC 1.14.13.39 antibody
    • IHPS 1 antibody
    • IHPS1 antibody
    • N NOS antibody
    • N-NOS antibody
    • NC NOS antibody
    • NC-NOS antibody
    • neuronal Nitric Oxide Synthase antibody
    • Neuronal NOS antibody
    • Nitric oxide synthase , neuronal, included antibody
    • Nitric oxide synthase 1 (neuronal) antibody
    • Nitric oxide synthase 1 antibody
    • Nitric oxide synthase brain antibody
    • Nitric oxide synthase, brain antibody
    • Nitric oxide synthase, penile neuronal, included antibody
    • nNOS antibody
    • NO antibody
    • NOS 1 antibody
    • NOS antibody
    • NOS type I antibody
    • NOS-I antibody
    • NOS1 antibody
    • NOS1_HUMAN antibody
    • Peptidyl-cysteine S-nitrosylase NOS1 antibody
    see all

Anti-nNOS (neuronal) (phospho S1417) antibody images

  • Immunocytochemistry/Immunofluorescence analysis of nNOS (neuronal) (phospho S1417) (green) showing staining in the cytoplasm and nucleus of SH-SY5Y cells treated with 100 ng/ml nocodazole (left) and untreated SH-SY5Y cells (right). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5583 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • ab5583 staining nNOS (phospho S1417) in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formalin, permeabilized with 0.25% Triton X-100 and blocked with 3% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/500 in PBS + Triton X-10 + normal donkey serum) for 48 hours at 4°C. A Biotin-conjugated Donkey anti-rabbit IgG polyclonal (1/10000) was used as the secondary antibody.

    See Abreview

References for Anti-nNOS (neuronal) (phospho S1417) antibody (ab5583)

This product has been referenced in:
  • Pereira VS  et al. Antidepressant- and anticompulsive-like effects of purinergic receptor blockade: involvement of nitric oxide. Eur Neuropsychopharmacol 23:1769-78 (2013). Read more (PubMed: 23434291) »
  • Garzón J  et al. RGSZ2 Binds to the Neural Nitric Oxide Synthase PDZ Domain to Regulate Mu-Opioid Receptor-Mediated Potentiation of the N-Methyl-D-Aspartate Receptor-Calmodulin-Dependent Protein Kinase II Pathway. Antioxid Redox Signal 15:873-87 (2011). WB ; Mouse . Read more (PubMed: 21348811) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 31 2013

Thank you for this information. Based on your protocol I think increasing the amount of primary antibody you use would improve your result. We recommend using 2 ug/ml, which would be 1/500. I would try using this (or even 1/250) overnight at 4 degrees....

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Application Western blot
Sample Mouse Cell lysate - whole cell (Not Given)
Loading amount 30 µg
Specification Not Given
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 04 2012

I am very pleased to hear you would like to use ab5583 in WB and send us an image of your results.

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview with the image for W...

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Thank you for your reply.


If you have purchased ab90443 within the last month and are able to submit data in ICC, I am able to issue a code retroactively. However, you will not be able to use this code to obtain ab5583 to test it. You mus...

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Thank you very much for your interest in ab5583 and ab90443.

We do not currently have an image of ab5583 in WB and ab90443 has not been tested in ICC. Therefore, I can offer a discount off a future purchase if you buyab5583 and/or ab90443 now,...

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Thank you for your telephone enquiry this afternoon. I am sorry to hear you have had difficulty obtaining satisfactory results from the current vial of this antibody.

I appreciate the time you have spent in the laboratory and understand your...

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Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Nov 24 2011

Application Western blot
Sample Human Tissue lysate - whole (Penile Tissue)
Loading amount 60 µg
Specification Penile Tissue
Gel Running Conditions Reduced Denaturing (4-15% mini gel)
Blocking step Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Miss. gwen Lagoda

Verified customer

Submitted Nov 18 2011

I am very pleased to hear you would like to accept our offer and test ab16650 and ab5583 in electric fish. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for electric fish and...

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