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Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Mouse nNOS (neuronal), phosphorylated at S847.
(Peptide available as ab16981.)
Our Abpromise guarantee covers the use of ab16650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).|
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab16650 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
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