Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab110352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
In-Cell ELISA Use a concentration of 4 µg/ml. 0.4 µg/well
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 114 kDa.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionThe transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.
  • Sequence similaritiesIn the N-terminal section; belongs to the AlaDH/PNT family.
    In the C-terminal section; belongs to the PNT beta subunit family.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GCCD4 antibody
    • mitochondrial antibody
    • NAD(P) transhydrogenase antibody
    • NAD(P) transhydrogenase mitochondrial antibody
    • Nicotinamide nucleotide transhydrogenase antibody
    • NNT antibody
    • NNTM antibody
    • NNTM_HUMAN antibody
    • Pyridine nucleotide transhydrogenase antibody
    see all

Anti-NNT antibody [8B4BB10] images

  • HL-60 cells were stained with 1 µg/mL NNT antibody (ab110352) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Mitochondrial localization of NNT in cultured, normal Human skin fibroblasts (strain HDFn) labeled with anti NNT (ab110352) as the primary antibody and an AlexaFluor® 594 goat anti-mouse IgG1 secondary antibody.
  • NNT immunohistochemistry in Human cerebellum visualized with ab110352 at 1/100. NNT immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
  • Nicotinamide nucleotide transhydrogenase (NNT) immunocaptured from Human liver mitochondria (lane 1), bovine heart mitochondria (lane 2), rat liver mitochondria (lane 3), mouse liver mitochondria (lane 4), and HepG2 cells (lane 5), using ab110352.
  • Anti-NNT antibody [8B4BB10] (ab110352) at 0.5 µg/ml + HepG2 whole cell lysate

    Predicted band size : 114 kDa

References for Anti-NNT antibody [8B4BB10] (ab110352)

This product has been referenced in:
  • Agarwal AR  et al. Short-term cigarette smoke exposure induces reversible changes in energy metabolism and cellular redox status independent of inflammatory responses in mouse lungs. Am J Physiol Lung Cell Mol Physiol 303:L889-98 (2012). WB ; Mouse . Read more (PubMed: 23064950) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Mouse Tissue lysate - other (Lung mitochondrion)
Specification Lung mitochondrion
Blocking step Casein-TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Amit Agarwal

Verified customer

Submitted Jul 18 2013

Thank you for following up on this query.

The staining displayed on the datasheet of the Anti-NNT antibody [8B4BB10] is not from an Abreview but from testing we have commissioned ourselves.

This was performed on human cerebellum tis...

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Thank you for contacting us.

I have had the opportunity to speak with the lab regarding to the antibody ab110352 [8B4BB10], we only tested human whole cell lysate (HepG2) not mouse cell lysate. This antibody has low affinity to mouse NNT b...

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I am sorry that you still did not receive a signal with the original antibody when using the positive control that I sent. I have processed you request to receive a new vial of ab110352 and you should have it by Friday of this week. I am sorry ...

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Thank you for confirming these details and for your cooperation. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order n...

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Thank you for contacting us and reporting this problem. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. The information provided by you will also allow us to investigate this case i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"