Overview

  • Product nameAnti-NNT1 antibody
    See all NNT1 primary antibodies
  • Description
    Rabbit polyclonal to NNT1
  • Tested applicationsSuitable for: ELISA, WB, Neutralising, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant NNT1/BCSF3 (Human).

Properties

  • FormLyophilised:Reconstitute with 100ul sterile distilled water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: None.
    Constituents: 0.5X PBS, pH 7.4.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was purified by chromatography.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.

Allows the detection of 0.2-0.4 ng/well of immunogen.

WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 25 kDa.

When used in conjunction with compatible secondary reagents the detection limit for this immunogen is 1.5 - 3.0 ng/lane, under either reducing or non reducing conditions.

Neutralising Use at an assay dependent concentration.
Sandwich ELISA 1/500. PubMed: 22319590

ab26125 has been used as the capture antibody in Pubmed 22319590.

Target

  • FunctionCytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor.
  • Tissue specificityExpressed predominantly in lymph nodes, spleen, peripheral blood lymphocytes, bone marrow, and fetal liver.
  • Involvement in diseaseDefects in CLCF1 are the cause of cold-induced sweating syndrome type 2 (CISS2) [MIM:610313]. Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high-arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell stimulating factor 3 antibody
    • B-cell-stimulating factor 3 antibody
    • BSF 3 antibody
    • BSF-3 antibody
    • BSF3 antibody
    • Cardiotrophin like cytokine antibody
    • Cardiotrophin like cytokine factor 1 antibody
    • Cardiotrophin-like cytokine factor 1 antibody
    • CISS 2 antibody
    • CISS2 antibody
    • CLC antibody
    • CLCF 1 antibody
    • Clcf1 antibody
    • CLCF1_HUMAN antibody
    • Cold induced sweating syndrome 2 antibody
    • CRLF 1 associated cytokine like factor 1 antibody
    • CRLF1 associated cytokine like factor 1 antibody
    • Neurotrophin 1 antibody
    • Neurotrophin 1/B cell stimulating factor 3 antibody
    • Neurotrophin1 antibody
    • NNT 1 antibody
    • NNT-1 antibody
    • Novel neurotrophin 1 antibody
    • Novel neurotrophin-1 antibody
    • NR 6 antibody
    • NR6 antibody
    see all

Anti-NNT1 antibody images

  • Measurement of NNT1 levels in conditioned media taken from 7 Human non-small cell lung carcinoma lines, using ab26125 as the capture antibody in a Sandwich ELISA.

    Conditioned media was collected from all 7 cell lines 48 hours after plating in serum-free media. A 96-well plate was coated with rabbit polyclonal to NNT1 (ab26125 at 1/500 in PBS) overnight at 4°C. 200 µL conditioned media was added to the plates and incubated overnight at 4°C. A mouse monoclonal against NNT1 was added followed by an HRP-conjugated goat anti-mouse secondary antibody. Absorbance was read at 450 nm.

References for Anti-NNT1 antibody (ab26125)

This product has been referenced in:
  • Looyenga BD  et al. STAT3 is activated by JAK2 independent of key oncogenic driver mutations in non-small cell lung carcinoma. PLoS One 7:e30820 (2012). Sandwich ELISA ; Human . Read more (PubMed: 22319590) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"