Highly pure (>98%) recombinant NNT1/BCSF3 (Human).
Lyophilised:Reconstitute with 100ul sterile distilled water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml.
Allows the detection of 0.2-0.4 ng/well of immunogen.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 25 kDa.
When used in conjunction with compatible secondary reagents the detection limit for this immunogen is 1.5 - 3.0 ng/lane, under either reducing or non reducing conditions.
Use at an assay dependent concentration.
1/500. PubMed: 22319590
ab26125 has been used as the capture antibody in Pubmed 22319590.
Cytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor.
Expressed predominantly in lymph nodes, spleen, peripheral blood lymphocytes, bone marrow, and fetal liver.
Involvement in disease
Defects in CLCF1 are the cause of cold-induced sweating syndrome type 2 (CISS2) [MIM:610313]. Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high-arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis.
Sandwich ELISA - Anti-NNT1 antibody (ab26125)Image from Looyenga BD et al., PLoS One. 2012;7(2):e30820. Epub 2012 Feb 2. Fig S2.; doi:10.1371/journal.pone.0030820; February 2, 2012, PLoS ONE 7(2): e30820.
Measurement of NNT1 levels in conditioned media taken from 7 Human non-small cell lung carcinoma lines, using ab26125 as the capture antibody in a Sandwich ELISA.
Conditioned media was collected from all 7 cell lines 48 hours after plating in serum-free media. A 96-well plate was coated with rabbit polyclonal to NNT1 (ab26125 at 1/500 in PBS) overnight at 4°C. 200 µL conditioned media was added to the plates and incubated overnight at 4°C. A mouse monoclonal against NNT1 was added followed by an HRP-conjugated goat anti-mouse secondary antibody. Absorbance was read at 450 nm.
Looyenga BD et al. STAT3 is activated by JAK2 independent of key oncogenic driver mutations in non-small cell lung carcinoma. PLoS One7:e30820 (2012).
Read more (PubMed: 22319590) »
Herholz J et al. Differential secretion of the mutated protein is a major component affecting phenotypic severity in CRLF1-associated disorders. Eur J Hum Genet19:525-33 (2011).
Read more (PubMed: 21326283) »