Overview

  • Product nameAnti-NOB1 antibody
    See all NOB1 primary antibodies
  • Description
    Rabbit polyclonal to NOB1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (EIRDKATRRR LAVLPYELRF KEPLPEYVRL VTEFSKKTGD YPSLSATDIQ) of human NOB1 (NP_054781).

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab87151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-NOB1 antibody images

  • Anti-NOB1 antibody (ab87151) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : ~22 kDa. We are unsure as to the identity of these extra bands.

References for Anti-NOB1 antibody (ab87151)

This product has been referenced in:
  • Li W  et al. Downregulated miR-646 in clear cell renal carcinoma correlated with tumour metastasis by targeting the nin one binding protein (NOB1). Br J Cancer 111:1188-200 (2014). WB . Read more (PubMed: 25010867) »
  • Zhou J  et al. MicroRNA-326 functions as a tumor suppressor in glioma by targeting the Nin one binding protein (NOB1). PLoS One 8:e68469 (2013). WB ; Human . Read more (PubMed: 23869222) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"