Overview

  • Product nameAnti-NOC2L antibody
    See all NOC2L primary antibodies
  • Description
    Rabbit polyclonal to NOC2L
  • Specificityab75047 detects endogenous levels of total NOC2L protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized peptide derived from an internal region of human NOC2L.

  • Positive control
    • Extracts from MCF-7 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab75047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 85 kDa.
ELISA 1/40000.

Target

  • FunctionActs as an inhibitor of histone acetyltransferase activity; prevents acetylation of all core histones by the EP300/p300 histone acetyltransferase at p53/TP53-regulated target promoters in a histone deacetylases (HDAC)-independent manner. Acts as a transcription corepressor of p53/TP53- and TP63-mediated transactivation of the p21/CDKN1A promoter. Involved in the regulation of p53/TP53-dependent apoptosis. Associates together with TP63 isoform TA*-gamma to the p21/CDKN1A promoter.
  • Sequence similaritiesBelongs to the NOC2 family.
  • Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus. Translocates from the nucleoli to the nucleoplasm in presence of several stressors like ultraviolet irradiation and actinomycin-D. Predominantly detected in the nucleoli in non-mitotic cells. Predominantly detected in nucleoplasma in cells undergoing mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • NIR antibody
    • NOC2 like antibody
    • NOC2-like protein antibody
    • NOC2L antibody
    • NOC2L_HUMAN antibody
    • Novel INHAT (inhibitor of histone acetyltransferase) repressor antibody
    • Novel INHAT repressor antibody
    • Nucleolar complex associated 2 homolog (S. cerevisiae) antibody
    • Nucleolar complex protein 2 homolog antibody
    • Protein NOC2 homolog antibody
    see all

Anti-NOC2L antibody images

  • All lanes : Anti-NOC2L antibody (ab75047) at 1/500 dilution

    Lane 1 : extracts from MCF-7 cells
    Lane 2 : extracts from MCF-7 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 85 kDa
    Observed band size : 85 kDa

References for Anti-NOC2L antibody (ab75047)

ab75047 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (gastric)
Loading amount 20 µg
Specification gastric
Gel Running Conditions Non-reduced Denaturing (4-12 % gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 19 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"