Anti-Nociceptin receptor antibody (ab66219)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Nociceptin receptor antibody
See all Nociceptin receptor primary antibodies -
DescriptionRabbit polyclonal to Nociceptin receptor
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Frmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
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Positive control
- Brain (singulate cortex)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Related Products
Applications
Our Abpromise guarantee covers the use of ab66219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/400. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).Can be blocked with Nociceptin receptor peptide (ab129205). | |
| IHC-Fr | 1/40 - 1/100. |
Target
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FunctionReceptor for the neuropeptide nociceptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 4987 Human
- Entrez Gene: 18389 Mouse
- Entrez Gene: 29256 Rat
- Omim: 602548 Human
- SwissProt: P41146 Human
- SwissProt: P35377 Mouse
- SwissProt: P35370 Rat
- Unigene: 2859 Human
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Alternative names
- H.sapiens mRNA for ORL1 receptor antibody
- Kappa type 3 opioid receptor antibody
- Kappa-type 3 opioid receptor antibody
see all
Anti-Nociceptin receptor antibody images
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Immunocytochemistry - Anti-Nociceptin receptor antibody (ab66219)Ab66219 staining of Oprl1 in rat brain (singulate cortex) by immunocytochemistry. -
Western blot - Anti-Nociceptin receptor antibody (ab66219)
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 41 kDaSk-N-SH cells were incubated at 37°C for 24h with vehicle control (0 µM) and 1 µM of kainic acid (ab120100). Decrease expression of nociceptin receptor in SK-N-SH cells correlates with an increase in kainic acid concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 30µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab66219 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
Protocols
References for Anti-Nociceptin receptor antibody (ab66219)
This product has been referenced in:
- Wheway G et al. An siRNA-based functional genomics screen for the identification of regulators of ciliogenesis and ciliopathy genes. Nat Cell Biol 17:1074-87 (2015). ICC/IF . Read more (PubMed: 26167768) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"