Anti-Nocturnin antibody (ab22850)

Overview

  • Product nameAnti-Nocturnin antibody
  • Description
    Rabbit polyclonal to Nocturnin
  • SpecificityAlthough there is a high homology with mouse, this antibody also has the potential to cross react with another mouse protein of similar molecular weight therefore this antibody is not suitable for use in mouse.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow
  • Immunogen

    A region within a synthetic peptide: RLYSALAKTL NSSAASQHPE YLVSPDPEHL EPIDPKELLE ECRAVLHTRP, corresponding to N terminal amino acids 73-122 of Human Nocturnin

  • Positive control
    • Human lung tissue (IHC-P), Daudi cell lysate (western blot).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • RelevanceCCRN4L is highly similar to Nocturnin, a gene identified as a circadian clock regulated gene in Xenopus laevis. This protein and Nocturnin protein share similarity with the C terminal domain of a yeast transcription factor, carbon catabolite repression 4 (CCR4). The mRNA abundance of a similar gene in mouse has been shown to exhibit circadian rhythmicity, which suggests a role for this protein in clock function or as a circadian clock effector.
  • Database links
  • Alternative names
    • CCR4 carbon catabolite repression 4 like (S. cerevisiae) antibody
    • CCR4 protein homolog antibody
    • CCRN4L antibody
    • NOC antibody

Anti-Nocturnin antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Nocturnin with ab22850 at 4.0-8.0µg/ml. Positive staining seen in the bronchiole epithelium. Magnification: 400X.
  • Anti-Nocturnin antibody (ab22850) at 4 µg/ml + Daudi cell lysate

    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Paraffin-embedded Human lung tissue. Cells with positive label: epithelial cells of bronchiole (indicated by arrows). Antibody concentration: 4-8µg/ml. Magnification: 400x.

References for Anti-Nocturnin antibody (ab22850)

ab22850 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (3T3 cells (fibroblasts))
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 20 µg
Specification 3T3 cells (fibroblasts)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Jun 10 2015

Thank you for getting back to me with further information. I have read your previous questionnaire and the further details that you have provided me with. I find your enquiry most interesting. As I can see you are ONLY detecting the correctly sized...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionnaire and I have a few comments. You mention that this antibody has been detecting bands other than ove...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"