Anti-Nodal antibody (ab39953)

Overview

  • Product name
  • Description
    Rabbit polyclonal to Nodal
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Nodal.

    (Peptide available as ab39952.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; MEF1; F9; Mouse EG; SHSY-5Y.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notes
    ICC/IF: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Essential for mesoderm formation and axial patterning during embryonic development.
    • Involvement in disease
      Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.
    • Sequence similarities
      Belongs to the TGF-beta family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • HTX5 antibody
      • MGC138230 antibody
      • Nodal antibody
      • Nodal Growth Differentiation Factor antibody
      • nodal homolog (mouse) antibody
      • Nodal homolog antibody
      • Nodal, Mouse, Homolog antibody
      • NODAL_HUMAN antibody
      see all

    Images

    • ICC/IF image of ab39953 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39953, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
    • All lanes : Anti-Nodal antibody (ab39953) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
      Lane 4 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate (ab27193)
      Lane 5 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
      Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to rabbit IgG -H&L - pre adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 40 kDa
      Observed band size : 40 kDa
      Additional bands at : 70 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    References

    ab39953 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for contacting Abcam.

    The immunogen information you requested is listed below for each antibody:
    1. ab39242: immunogen is upstream of residue XXX.
    2. ab39953: immunogen is downstream of residue XXX.
    3. ab109317: immunogen...

    Read More
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (whole embryo E6.5)
    Specification
    whole embryo E6.5
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - PBtriton-x-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 110% · Temperature: RT°C
    Username

    Dr. T Rodriguez

    Verified customer

    Submitted Aug 14 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up