• Product name
  • Description
    Mouse monoclonal to Nodal
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: RCEGECPNPV GEEFHPTNHA YIQSLLKRYQ PHRVPSTCCA PVKTKPLSML YVDNGRVLLD HHKDMIVEEC GC, corresponding to amino acids 275-347 of Human Nodal

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
  • Isotype
  • Light chain type
  • Research areas


Our Abpromise guarantee covers the use of ab55676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • Function
    Essential for mesoderm formation and axial patterning during embryonic development.
  • Involvement in disease
    Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • HTX5 antibody
    • MGC138230 antibody
    • Nodal antibody
    • Nodal Growth Differentiation Factor antibody
    • nodal homolog (mouse) antibody
    • Nodal homolog antibody
    • Nodal, Mouse, Homolog antibody
    • NODAL_HUMAN antibody
    see all


  • Nodal antibody (ab55676) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium cancer.

  • Predicted band size : 40 kDa
    Nodal antibody (ab55676) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ICC/IF image of ab55676 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55676, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HEK293 cells stained with ab55676 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55676, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.


This product has been referenced in:
  • Bodenstine TM  et al. Nodal expression in triple-negative breast cancer: Cellular effects of its inhibition following doxorubicin treatment. Cell Cycle 15:1295-302 (2016). Read more (PubMed: 27007464) »
  • Margaryan NV  et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci 17:418 (2016). IHC . Read more (PubMed: 27011171) »

See all 10 Publications for this product

Customer reviews and Q&As

Thank you for your reply.

I am sorry about the confusion whether ab55676 was tested in IF or not, as you say the image on the datasheet clearly shows that is does work in IF, I am sorry I missed that.

Having, looked over your protoc...

Read More
Immunocytochemistry/ Immunofluorescence
Human Cell (1 week Retinoic acid differentiation of NTERA-2)
1 week Retinoic acid differentiation of NTERA-2
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Oct 21 2015

Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (12)
Human Cell lysate - whole cell (LNCaP cell line)
LNCaP cell line
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

Dr. Francesco Elia Marino

Verified customer

Submitted May 15 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
CAS BLOCK as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 26°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer
Human Tissue sections (Human Prostate)
Human Prostate

Dr. Francesco Elia Marino

Verified customer

Submitted May 15 2014

Thank you for your reply.

I am sorry that I misread your original email with the protocol information, I know see that you tried the secondary antibody with both 15 and 30 minutes incubation times but that did not make a difference.

Read More

Thank you for taking the time to respond and for contacting Abcam.

With over 70,000 products it is logistically impossible for us to offer free samples. I hope you understand.

Please let me know if you have any questions.

Thank you for contacting Abcam.

I am sorry that you are having problems with ab55676 in immunofluorescence. As we had not tested this antibody in IF before, we were not sure how the antibody would work in that application. I would be very hap...

Read More

Thank you for contacting Abcam.

I am sorry to hear that Ab55676 has not performed well for you. Although the peptide sequence has excellent identity with the mouse homolog, this product is not guaranteed to work in mouse tissues. High backgro...

Read More

Further to my previous response to your complaint, I would like to pass some more information which you may find useful. The antibody is (NODAL monoclonal antibody (ab55676) has been tested and characterized in Western blot on several cell lines, such ...

Read More

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. NODAL is essential for mesoderm formation and axial pat...

Read More

1-10 of 11 Abreviews or Q&A


Sign up