Recombinant
RabMAb

Anti-Nodal antibody [EP2058Y] (ab81287)

Overview

  • Product name
    Anti-Nodal antibody [EP2058Y]
    See all Nodal primary antibodies
  • Description
    Rabbit monoclonal [EP2058Y] to Nodal
  • Specificity
    ab81287 detects Pro-Nodal and Nodal.
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of human Nodal.

  • Positive control
    • Recombinant nodal protein.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab81287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/2000. Predicted molecular weight: 39 kDa. (Pro-Nodal) and 13 kDa (Nodal).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Essential for mesoderm formation and axial patterning during embryonic development.
    • Involvement in disease
      Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.
    • Sequence similarities
      Belongs to the TGF-beta family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • HTX5 antibody
      • MGC138230 antibody
      • Nodal antibody
      • Nodal Growth Differentiation Factor antibody
      • nodal homolog (mouse) antibody
      • Nodal homolog antibody
      • Nodal, Mouse, Homolog antibody
      • NODAL_HUMAN antibody
      see all

    Anti-Nodal antibody [EP2058Y] images

    • Anti-Nodal antibody [EP2058Y] (ab81287) at 1/2000 dilution + Recombinant nodal protein at 10ng

      Secondary
      HRP conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 39 kDa
      Observed band size : 15 kDa (why is the actual band size different from the predicted?)

    References for Anti-Nodal antibody [EP2058Y] (ab81287)

    This product has been referenced in:
    • Strizzi L  et al. Effects of a novel Nodal-targeting monoclonal antibody in melanoma. Oncotarget 6:34071-86 (2015). Sandwich ELISA ; Human . Read more (PubMed: 26460952) »
    • Malchenko S  et al. Cancer hallmarks in induced pluripotent cells: new insights. J Cell Physiol 225:390-3 (2010). WB ; Human . Read more (PubMed: 20568225) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting Abcam.

    The immunogen for ab81287 is downstream of Nodal residue XXX.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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