Anti-Nodal antibody [EPR2057] (ab109317)


  • Product name
    Anti-Nodal antibody [EPR2057]
    See all Nodal primary antibodies
  • Description
    Rabbit monoclonal [EPR2057] to Nodal
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Nodal.

  • Positive control
    • WB: SH-SY5Y and PC-12 cell lysates Flow Cyto: Permeabilized SH-SY5Y cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab109317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Essential for mesoderm formation and axial patterning during embryonic development.
    • Involvement in disease
      Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.
    • Sequence similarities
      Belongs to the TGF-beta family.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • HTX5 antibody
      • MGC138230 antibody
      • Nodal antibody
      • Nodal Growth Differentiation Factor antibody
      • nodal homolog (mouse) antibody
      • Nodal homolog antibody
      • Nodal, Mouse, Homolog antibody
      • NODAL_HUMAN antibody
      see all

    Anti-Nodal antibody [EPR2057] images

    • All lanes : Anti-Nodal antibody [EPR2057] (ab109317) at 1/1000 dilution

      Lane 1 : SH-SY5Y lysate
      Lane 2 : PC12 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 40 kDa
    • Flow cytometric analysis of Nodal in permeabilized SH-SY5Y cells, using ab109317 (red) or a rabbit IgG (negative) (green).

    References for Anti-Nodal antibody [EPR2057] (ab109317)

    This product has been referenced in:
    • Chen J  et al. Embryonic morphogen nodal is associated with progression and poor prognosis of hepatocellular carcinoma. PLoS One 9:e85840 (2014). WB ; Human . Read more (PubMed: 24465741) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting Abcam.

    The immunogen information you requested is listed below for each antibody:
    1. ab39242: immunogen is upstream of residue XXX.
    2. ab39953: immunogen is downstream of residue XXX.
    3. ab109317: immunogen...

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