Anti-Nodal antibody [EPR2057] (ab109317)


  • Product nameAnti-Nodal antibody [EPR2057]
    See all Nodal primary antibodies
  • Description
    Rabbit monoclonal [EPR2057] to Nodal
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Nodal.

  • Positive control
    • WB: SH-SY5Y and PC-12 cell lysates Flow Cyto: Permeabilized SH-SY5Y cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab109317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    • FunctionEssential for mesoderm formation and axial patterning during embryonic development.
    • Involvement in diseaseDefects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.
    • Sequence similaritiesBelongs to the TGF-beta family.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • HTX5 antibody
      • MGC138230 antibody
      • Nodal antibody
      • Nodal Growth Differentiation Factor antibody
      • nodal homolog (mouse) antibody
      • Nodal homolog antibody
      • Nodal, Mouse, Homolog antibody
      • NODAL_HUMAN antibody
      see all

    Anti-Nodal antibody [EPR2057] images

    • All lanes : Anti-Nodal antibody [EPR2057] (ab109317) at 1/1000 dilution

      Lane 1 : SH-SY5Y lysate
      Lane 2 : PC12 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 40 kDa
    • Flow cytometric analysis of Nodal in permeabilized SH-SY5Y cells, using ab109317 (red) or a rabbit IgG (negative) (green).

    References for Anti-Nodal antibody [EPR2057] (ab109317)

    This product has been referenced in:
    • Chen J  et al. Embryonic morphogen nodal is associated with progression and poor prognosis of hepatocellular carcinoma. PLoS One 9:e85840 (2014). WB ; Human . Read more (PubMed: 24465741) »

    See 1 Publication for this product

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    The immunogen information you requested is listed below for each antibody:
    1. ab39242: immunogen is upstream of residue XXX.
    2. ab39953: immunogen is downstream of residue XXX.
    3. ab109317: immunogen...

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