Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Noggin.
(Peptide available as ab16380.)
Our Abpromise guarantee covers the use of ab16054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/175. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 26, 35 kDa (predicted molecular weight: 26 kDa).Can be blocked with Human Noggin peptide (ab16380).|
ab16054 stainning Noggin in paraffin-embedded human liver tissue, showing a cytoplasmic and/or membranous distribution in both hepatocytes and bile duct cells. Paraffin embedded tissue was incubated with ab16054 (1/175 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab16054 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Immunohistochemical analysis of human small intestine tissue, labeling Noggin with ab16054. Tissue was formaldehyde fixed, treated with EDTA (pH 8.6) at 100°C for 20 minutes for heat-mediated antigen retrieval and blocked with 3% Hydrogen Peroxide for 10 minutes at 25°C. Incubation with ab16054 (diluted 1/400) was performed for 20 minutes at 25°C.
Immunohistochemical analysis of mouse kidney tissue, labeling Noggin with ab16054. Tissue was paraformaldehyde fixed, treated with Citrate buffer for heat-mediated antigen retrieval and blocked with Serum Free Protein Block for 20 minutes. Incubation with ab16054 (diluted 1/2500) was performed for 15 hours at 4°C