The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 6 µg/ml. Recommended: Steamed antigen retrieval with citrate buffer pH 6.
FunctionDevelopmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.
Tissue specificityIsoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific.
Sequence similaritiesContains 1 reticulon domain.
DomainThree regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo-66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor.
Cellular localizationEndoplasmic reticulum membrane. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
Neuroendocrine-specific protein C homolog antibody
Nogo A antibody
Nogo protein antibody
Reticulon 4 antibody
reticulon 5 antibody
RTN 4 antibody
RTN 4A antibody
RTN xL antibody
Anti-Nogo A antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nogo A antibody (ab107522)
Cytoplasm staining of Nogo A in neuronal cell bodies and in axons of paraffin embedded Human cortex by Immunohistochemistry, using ab107522 at a concentration of 2.5 µg/ml. Steamed antigen retrieval with citrate buffer
pH 6 and AP staining were used.
References for Anti-Nogo A antibody (ab107522)
has not yet been referenced specifically in any publications.
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