Overview

  • Product name
  • Description
    Goat polyclonal to Nogo A
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide raised against an internal region of Human Nogo A conjugated to KLH.

  • Positive control
    • Human Brain cortex, and testis tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab110110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.
  • Tissue specificity
    Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific.
  • Sequence similarities
    Contains 1 reticulon domain.
  • Domain
    Three regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo-66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor.
  • Cellular localization
    Endoplasmic reticulum membrane. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • ASY antibody
    • Foocen antibody
    • Human NogoA antibody
    • My043 protein antibody
    • Nbla00271 antibody
    • Nbla10545 antibody
    • neurite growth inhibitor 220 antibody
    • Neurite outgrowth inhibitor antibody
    • Neuroendocrine-specific protein antibody
    • Neuroendocrine-specific protein C homolog antibody
    • NI220/250 antibody
    • Nogo A antibody
    • NOGO antibody
    • Nogo protein antibody
    • NOGO-A antibody
    • Nogo-B antibody
    • Nogo-C antibody
    • NSP antibody
    • NSP-CL antibody
    • Reticulon 4 antibody
    • reticulon 5 antibody
    • Reticulon-4 antibody
    • Reticulon-5 antibody
    • RTN 4 antibody
    • RTN 4A antibody
    • RTN xL antibody
    • RTN-x antibody
    • Rtn4 antibody
    • RTN4-A antibody
    • RTN4-B1 antibody
    • RTN4-B2 antibody
    • RTN4-C antibody
    • RTN4_HUMAN antibody
    see all

Images

  • ab110110 at 2.5 µg/ml staining Nogo A in Formalin-Fixed, Paraffin-Embedded Human Brain cortex. Slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • ab110110 at 2.5 µg/ml staining Nogo A in Formalin-Fixed, Paraffin-Embedded Human Testis. Slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References

ab110110 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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