Overview

  • Product nameAnti-Nogo A+B antibody
  • Description
    Rabbit polyclonal to Nogo A+B
  • Specificityab47085 recognises Nogo A and Nogo B2 and B1
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, African Green Monkey
  • Immunogen

    Synthetic peptide, sequence taken from within amino acids 1-50 of human Nogo B.

  • Positive control
    • Human, mouse and rat brain tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab47085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250.
ICC/IF 1/200.
WB 1/500 - 1/2000. Detects a band of approximately >200 kDa.

Target

  • RelevanceNOGO is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Adult mammalian axon regeneration is generally successful in the peripheral nervous system but poor in the central nervous system. Inhibition results from physical barriers imposed by glial scars, a lack of neurotrophic factors, and growth-inhibitory molecules associated with myelin, the insulating axon sheath. These molecules include NI35, myelin-associated glycoprotein (159460), and Nogo. Several isoforms (A-E) of NOGO exist.
  • Cellular localizationEndoplasmic reticulum; endoplasmic reticulum membrane; multi-pass membrane protein. Note=Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
  • Database links
  • Alternative names
    • Nogo A antibody
    • Nogo antibody
    • Nogo B antibody
    • NSP antibody
    • Reticulon 4 antibody
    • Reticulon 5 antibody
    • RTN x antibody
    • RTN4 antibody
    • RTN4A antibody
    • RTNX antibody
    see all

Anti-Nogo A+B antibody images

  • All lanes : Anti-Nogo A+B antibody (ab47085) at 1/2000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Rat brain tissue lysate


    Observed band size : 41,43,48-50,>200 kDa (why is the actual band size different from the predicted?)
  • ab47085 staining Nogo A+B in African Green Monkey COS-7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/500 in PBS-BSA) for 1 hour at 23°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry stainning for Nogo A+B in Cor1 neural stem cells from mouse using Rabbit polyclonal to Nogo A+B (ab47085; 1/200 incubated for 2h at RT); Immunoreactivity was detected in cell-body as well as in neurites.

References for Anti-Nogo A+B antibody (ab47085)

This product has been referenced in:
  • Ortega A  et al. Endoplasmic reticulum stress induces different molecular structural alterations in human dilated and ischemic cardiomyopathy. PLoS One 9:e107635 (2014). WB ; Human . Read more (PubMed: 25226522) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Sample African Green Monkey Cell (COS-7)
Specification COS-7
Permeabilization Yes - 0.5% Triton-X
Fixative Paraformaldehyde
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Abcam user community

Verified customer

Submitted Oct 25 2013

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (12% gel)
Sample Mouse Cell lysate - whole cell (Mouse cardiac fibroblasts)
Specification Mouse cardiac fibroblasts
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
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Abcam user community

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Submitted May 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HELA)
Loading amount 50 µg
Specification HELA
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
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Abcam user community

Verified customer

Submitted Sep 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF)
Loading amount 50 µg
Specification MEF
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
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Abcam user community

Verified customer

Submitted Sep 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (MEF)
Specification MEF
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Sep 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HELA)
Specification HELA
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
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Abcam user community

Verified customer

Submitted Sep 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Gastrocnemius muscle)
Loading amount 100 µg
Specification Gastrocnemius muscle
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Barney Bryson

Verified customer

Submitted Jun 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Cor1 neural stem cells)
Specification Cor1 neural stem cells
Fixative Formaldehyde
Permeabilization Yes - 0.1% Tween
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Feb 10 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Forebrain: region of corpus callosum/striatum, cor)
Specification Forebrain: region of corpus callosum/striatum, cor
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"