A synthetic peptide corresponding to amino acids 64-74 (RNRRHHRHPRG) of human Nogo-B receptor (NgBR) was used as immunogen. This immunogen sequence is within the 93 amino acid putative ectodomain of the NgBR.
Human, mouse and rat brain tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 32 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActs as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator. Regulates the stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol. Essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the UPP synthase family.
Cellular localizationEndoplasmic reticulum membrane. Colocalizes with Nogo-B during VEGF and wound healing angiogenesis.