Anti-Nogo Receptor antibody (ab32890)

Overview

  • Product nameAnti-Nogo Receptor antibody
    See all Nogo Receptor primary antibodies
  • Description
    Goat polyclonal to Nogo Receptor
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:

    LQALP DDTFRDLGN

    , corresponding to Internal sequence amino acids 166-179 of Human Nogo Receptor

  • Positive control
    • Human brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab32890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Antibody detection limit dilution 1/16,000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 53 kDa. Gave bands at approx 90-100kDa and 37+40+45kDa in Human brain lysate and were successfully blocked by the immunizing peptide corresponding to earlier findings in literature (Wang et al).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Nogo Receptor antibody images

    • All lanes : Anti-Nogo Receptor antibody (ab32890) at 0.3 µg/ml

      Lane 1 : [A] Human brain lysate in RIPA buffer, without immunising peptide
      Lane 2 : [B] Human brain lysate in RIPA buffer, with immunising peptide

      Lysates/proteins at 35 µg per lane.

      Developed using the ECL technique

      Predicted band size : 53 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa,23 kDa,37 kDa,40 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-Nogo Receptor antibody (ab32890)

    ab32890 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    I can confirm that both products are tested and guaranteed for WB in Human samples. That means, if the customer would experience any difficulties we are pleased to help and offer some refund in case the product doe...

    Read More

    Thank you for contacting us. The concentration of this antibody is 0.5 mg/ml. This information can be found about halfway down the datasheet.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advic...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"