The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Antibody detection limit dilution 1/16,000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 53 kDa. Gave bands at approx 90-100kDa and 37+40+45kDa in Human brain lysate and were successfully blocked by the immunizing peptide corresponding to earlier findings in literature (Wang et al).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Nogo Receptor mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system. It is also a receptor for Nogo A (RTN4), OMG and MAG.