Recombinant Anti-NOL11 antibody [EPR14074-30] (ab181996)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14074-30] to NOL11
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NOL11 antibody [EPR14074-30]
See all NOL11 primary antibodies -
Description
Rabbit monoclonal [EPR14074-30] to NOL11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Hela, A431 and HepG2 cell lines.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14074-30 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
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ICC/IF |
1/500.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa). |
ICC/IF
1/500. |
Target
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Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 25926 Human
- SwissProt: Q9H8H0 Human
- Unigene: 463936 Human
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Alternative names
- NOL11 antibody
- NOL11_HUMAN antibody
- Nucleolar protein 11 antibody
Images
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All lanes : Anti-NOL11 antibody [EPR14074-30] (ab181996) at 1/5000 dilution
Lane 1 : Hela cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 81 kDa -
Immunofluorescent analysis of -20℃ acetone-fixed Hela cell line staining NOL11 using ab181996 at a 1/500 dilution (red). Goat anti rabbit IgG (Dylight 555) at a 1/200 dilution was used as a secondary antibody. Counter stain: Dapi (blue).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cell line staining NOL11 using ab181996 at a 1/500 dilution (red). Goat anti rabbit IgG (Dylight 555) at a 1/200 dilution was used as a secondary antibody. Counter stain: Dapi (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181996 has not yet been referenced specifically in any publications.