Recombinant
RabMAb

Anti-NOL11 antibody [EPR14074-30] (ab181996)

Overview

  • Product name
    Anti-NOL11 antibody [EPR14074-30]
    See all NOL11 primary antibodies
  • Description
    Rabbit monoclonal [EPR14074-30] to NOL11
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NOL11 aa 500-650. The exact sequence is proprietary.
    Database link: Q9H8H0

  • Positive control
    • Hela, A431 and HepG2 cell lines.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14074-30
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
ICC/IF 1/500.

Target

Images

  • All lanes : Anti-NOL11 antibody [EPR14074-30] (ab181996) at 1/5000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 81 kDa

  • Immunofluorescent analysis of -20℃ acetone-fixed Hela cell line staining NOL11 using ab181996 at a 1/500 dilution (red). Goat anti rabbit IgG (Dylight 555) at a 1/200 dilution was used as a secondary antibody. Counter stain: Dapi (blue).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cell line staining NOL11 using ab181996 at a 1/500 dilution (red). Goat anti rabbit IgG (Dylight 555) at a 1/200 dilution was used as a secondary antibody. Counter stain: Dapi (blue).

References

ab181996 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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