Overview

  • Product nameAnti-NOL6 antibody
    See all NOL6 primary antibodies
  • Description
    Rabbit polyclonal to NOL6
  • SpecificityThis antibody reacts with NOL6
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    A region within synthetic peptide: VIGVLWKPTS FQPQPFKASS TKGRMVMSRG GELVMVPNVE AILEDFAVLG, corresponding to C terminal amino acids 1081-1130 of Human NOL6

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab50875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:1562500.

WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 22 , 128 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 16 µg/ml.

Target

  • RelevanceNOL6 is a nucleolar RNA associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts.
  • Cellular localizationNucleus; nucleolus. Localizes to condensed chromosomes in mitosis
  • Database links
  • Alternative names
    • bA311H10.1 antibody
    • FLJ21959 antibody
    • MGC14896 antibody
    • MGC14921 antibody
    • MGC20838 antibody
    • NRAP antibody
    • Nucleolar protein 6 antibody
    • Nucleolar protein family 6 (RNA associated) antibody
    • Nucleolar RNA associated protein antibody
    • UTP22 antibody
    see all

Anti-NOL6 antibody images

  • Lane 1 :
    Lane 2 : Anti-NOL6 antibody (ab50875) at 2.5 µg/ml

    Lane 1 : MARKER
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 22 , 128 kDa
  • ab50875 at 16µg/ml staining NOL6 in human epithelial cells of intestinal villus.

References for Anti-NOL6 antibody (ab50875)

ab50875 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"