Overview

  • Product nameAnti-NOL8 antibody
  • Description
    Rabbit polyclonal to NOL8
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 200 and 250 of human NOL8 (NP_060418.4).

  • Positive control
    • HeLa, 293T cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab95898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 132 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionPlays an essential role in the survival of diffuse-type gastric cancer cells. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells.
  • Tissue specificityExpressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.
  • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C9orf34 antibody
    • NOL8 antibody
    • NOL8_HUMAN antibody
    • NOP132 antibody
    • Nucleolar protein 8 antibody
    • Nucleolar protein Nop132 antibody
    see all

Anti-NOL8 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling NOL8 with ab95898 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-NOL8 antibody (ab95898) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 132 kDa


    Exposure time : 30 seconds
  • Detection of human NOL8 by Western Blot of Immunoprecipitate. ab95898 at 0.1µg/ml staining NOL8 in HeLa cell lysate immunoprecipitated using ab95898 at 3µg/mg (1mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

References for Anti-NOL8 antibody (ab95898)

ab95898 has not yet been referenced specifically in any publications.

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