1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Plays an essential role in the survival of diffuse-type gastric cancer cells. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells.
Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling NOL8 with ab95898 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - NOL8 antibody (ab95898)
All lanes : Anti-NOL8 antibody (ab95898) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 132 kDa
Exposure time: 30 seconds
Immunoprecipitation - NOL8 antibody (ab95898)
Detection of human NOL8 by Western Blot of Immunoprecipitate.
ab95898 at 0.1µg/ml staining NOL8 in HeLa cell lysate immunoprecipitated using ab95898 at 3µg/mg (1mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.