Western blot - non-muscle Myosin IIA antibody (ab24762)

Anti-non-muscle Myosin IIA antibody (ab24762) at 1/1000 dilution + Rat PC12 whole cell lysate at 20 µg

Secondary
HRP conjugated swine anti-rabbit
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 226 kDa
Observed band size : 220 kDa (why is the actual band size different from the predicted?)


Exposure time : 2 minutes

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence - non-muscle Myosin IIA antibody (ab24762)

ab24762 staining non-muscle Myosin IIA in Human fibrosarcoma cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-10. Samples were incubated with 10 µg/ml primary antibody in PBS for 1 hour at 20°C. An Alexa Fluor^® 555-conjugated Donkey polyclonal to rabbit IgG, dilution 1/500, was used as secondary antibody. Nuclei were counterstained with DAPI.

This image is courtesy of an anonymous Abreview

Flow Cytometry - non-muscle Myosin IIA antibody (ab24762)

ab24762 detecting non-muscle Myosin IIA in Human platelets by Flow Cytometry. Platelets were isolated by spinning Platelet rich plasma on Histopaque. Cells were fixed in PFA and permeabilized in 0.1% Triton-X100 in 2% BSA for 30 minutes. The primary antibody was diluted 1/200 in 2% BSA in PBS and incubated with the sample for 18 hours at 4ºC. An Alexa Fluor® 488-conjugated Chicken anti-Rabbit IgG (H+L) antibody, diluted 1/500 was used as the secondary antibody.

Abbreviations in the figure:
US Unstained, Red Peak;
IgG Rb: IgG Rabbit (Isotype Control), Blue Peak;
Myosin: Myosin IIA, Green Peak.

This image is courtesy of an Abreview by Mahesh Shivananjappa.

Immunocytochemistry/ Immunofluorescence - non-muscle Myosin IIA antibody (ab24762)

ab24762 staining non-muscle Myosin IIA in Human embryonic stem cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde,permeabilized with 0.5% Triton X-100 and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/400 in PBS + 0.05% Tween20) for 1 hour at room temperature. A DyLight^® 488-conjugated Donkey polyclonal to rabbit IgG, dilution 1/200, was used as the secondary antibody.

This image is courtesy of an anonymous Abreview