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Western blot - non-muscle Myosin IIA antibody (ab24762)
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Western blot - non-muscle Myosin IIA antibody (ab24762)
(enlarge)
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Immunocytochemistry/ Immunofluorescence - non-muscle Myosin IIA antibody (ab24762)
(enlarge)
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Product Name
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non-muscle Myosin IIA antibody
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See all Myosin IIA (non muscle) antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to non-muscle Myosin IIA
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Immunogen
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Synthetic peptide: GKADGAEAKPAE, corresponding to C terminal amino acids 1948/1959 of Human non-muscle Myosin IIA
BLAST 'GKADGAEAKPAE' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat, Cow, Hm
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Epitope
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ab24762 reacts with an epitope located in the C terminus of Myosin IIA.
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Tested applications
(see key)
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ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml (see Abreview).
IF: 1/500.
IHC-P: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/1000. Predicted molecular weight: 226 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin
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Relevance
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Myosin IIA (non-muscle) is a relatively abundant, widespread two headed myosin composed of an N-terminal motor domain, a light chain binding neck region, a coiled-coil region, and a non helical C-terminal domain. It forms a heterohexamer composed of a pair of heavy chains and two pairs of light chains. Non-muscle myosin II is involved in cell motility and adhesion, cytokinesis, vesicular transport, intracellular force generation, and in morphogenesis during development.
Mutations in the Non-muscle Myosin IIA (MYH9) gene result in Sebastian platelet syndrome, May-Hegglin anomaly, Fechtner and Epstein syndromes.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.03% Thimerosal (merthiolate)
Constituents: TBS Material safety datasheet (MSDS) for this product:
Thimerosal MSDS
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Purification notes
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ab24762 is an affinity purified IgG.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this non-muscle Myosin IIA antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for non-muscle Myosin IIA antibody images, references, products related to ab24762 and other tools.
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non-muscle Myosin IIA antibody images:
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 Western blot - non-muscle Myosin IIA antibody (ab24762)
non-muscle Myosin IIA antibody (ab24762) at 1/1000 dilution + Human HeLa S3 - whole cell lysate at 20 µg
Secondary
HRP conjugated pig anti-rabbit
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 226 kDa
Observed band size : 220 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes This image is courtesy of an anonymous Abreview
See Abreview
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Western blot - non-muscle Myosin IIA antibody (ab24762)
non-muscle Myosin IIA antibody (ab24762) at 1/1000 dilution + Rat PC12 whole cell lysate at 20 µg
Secondary
HRP conjugated swine anti-rabbit
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 226 kDa
Observed band size : 220 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutes This image is courtesy of an anonymous Abreview
See Abreview
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Immunocytochemistry/ Immunofluorescence - non-muscle Myosin IIA antibody (ab24762)
ab24762 staining non-muscle Myosin IIA in Human fibrosarcoma cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-10. Samples were incubated with 10 µg/ml primary antibody in PBS for 1 hour at 20°C. An Alexa Fluor® 555-conjugated Donkey polyclonal to rabbit IgG, dilution 1/500, was used as secondary antibody. Nuclei were counterstained with DAPI. This image is courtesy of an anonymous Abreview
See Abreview
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References for non-muscle Myosin IIA antibody (ab24762)
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This product has been used in: (two most recent references)
Maeda J et al. Proteomic analysis of stage I primary lung adenocarcinoma aimed at individualisation of postoperative therapy. Br J Cancer 98:596-603 (2008). PubMed: 18212748
Ponsaerts R et al. The myosin II ATPase inhibitor blebbistatin prevents thrombin-induced inhibition of intercellular calcium wave propagation in corneal endothelial cells. Invest Ophthalmol Vis Sci 49:4816-27 (2008). WB; Cow. PubMed: 18614806
See all 3 publication references for this product.
If you publish research using ab24762 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to non-muscle Myosin IIA
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non-muscle Myosin IIA antibody - more information
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Customer reviews (feedback) regarding non-muscle Myosin IIA antibody |
Customer FAQs regarding non-muscle Myosin IIA antibody |
Protocols for non-muscle Myosin IIA antibody |
Price and availability of products related to non-muscle Myosin IIA antibody |
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non-muscle Myosin IIA antibody - related products:
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non-muscle Myosin IIA antibody - other tools:
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Contact Abcam with a Technical Enquiry about non-muscle Myosin IIA antibody |
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