Anti-non-muscle Myosin IIB antibody [3H2] (ab684)

Overview

  • Product nameAnti-non-muscle Myosin IIB antibody [3H2]See all non-muscle Myosin IIB primary antibodies ...
  • Description
    Mouse monoclonal [3H2] to non-muscle Myosin IIB
  • SpecificityThis antibody reacts with non-muscle myosin heavy chain (SMemb).
  • Tested applicationsIHC-P, ICC/IF, WB, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Hamster, Human
  • Immunogen

    Synthetic peptide:

    TSDVNETQPPQSE

    , corresponding to C (beta) terminal amino acids 1964-1976 of Human non-muscle Myosin IIB.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferAscitic fluid
  • Concentration information loading...
  • PurityAscites
  • Clonality Monoclonal
  • Clone number3H2
  • IsotypeIgG2b
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
ICC/IF Use at an assay dependent dilution. PubMed: 17478566Use at an assay dependent dilution.
WB 1/2000.
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use 1µg for 106 cells.

Target

  • FunctionCellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificityIsoform 1 is expressed in cerebellum and spinal chord. Isoform 2 is expressed in cerebrum and retina. Isoform 3 is expressed in the cerebrum and to a much lower extent in cerebellum.
  • Sequence similaritiesContains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
  • Post-translational
    modifications
    Phosphorylated by ABL2.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cellular myosin heavy chain antibody
    • Cellular myosin heavy chain type B antibody
    • Cellular myosin heavy chain type B type B antibody
    • MGC134913 antibody
    • MGC134914 antibody
    • MYH 10 antibody
    • Myh10 antibody
    • MYH10_HUMAN antibody
    • Myosin 10 antibody
    • Myosin heavy chain 10 antibody
    • Myosin heavy chain 10 non muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain non muscle 11b antibody
    • Myosin heavy chain nonmuscle IIb antibody
    • Myosin heavy chain nonmuscle type B antibody
    • Myosin heavy polypeptide 10 non muscle antibody
    • Myosin-10 antibody
    • Myosin10 antibody
    • NMMHC B antibody
    • NMMHC II b antibody
    • NMMHC II-b antibody
    • NMMHC IIB antibody
    • NMMHC-B antibody
    • NMMHC-IIB antibody
    • NMMHCB antibody
    • Non muscle myosin heavy chain B antibody
    • Non muscle myosin heavy chain IIB antibody
    • Non muscle myosin II heavy chain B antibody
    • non-muscle IIb antibody
    • Non-muscle myosin heavy chain B antibody
    • Non-muscle myosin heavy chain IIb antibody
    • Nonmuscle myosin heavy chain B antibody
    • Nonmuscle myosin heavy chain IIB antibody
    • Nonmuscle myosin II heavy chain B antibody
    • type B antibody
    see all

Anti-non-muscle Myosin IIB antibody [3H2] images

  • ICC/IF image of ab684 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab684, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
  • Ab684 staining nnon-muscle Myosin IIB in Human colon.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Overlay histogram showing HeLa cells stained with ab684 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab684, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

References for Anti-non-muscle Myosin IIB antibody [3H2] (ab684)

This product has been referenced in:
  • Senol O  et al. miR-200a-mediated suppression of non-muscle heavy chain IIb inhibits meningioma cell migration and tumor growth in vivo. Oncogene 0:N/A (2014). WB . Read more (PubMed: 24858044) »
  • Rodríguez-Calvo R  et al. Over-expression of Neuron-derived Orphan Receptor-1 (NOR-1) exacerbates neointimal hyperplasia after vascular injury. Hum Mol Genet 22:1949-59 (2013). Mouse . Read more (PubMed: 23390133) »

See all 12 Publications for this product

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (4-12% SDS-PAGE)
Sample Human Cell lysate - other (293T)
Specification 293T
Treatment siRNA transfected for 2 days
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 15 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Aorta)
Specification Aorta
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6
Permeabilization No
Blocking step Blocking reagent from ab127055 kit as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Miss. Cristina Rodriguez

Verified customer

Submitted Apr 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (VSMC)
Loading amount 25 µg
Specification VSMC
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Miss. Cristina Rodriguez

Verified customer

Submitted Apr 12 2013

Leider bieten wir diesen Antikörper nicht in der konjugierten From an. Dies hat mehrere Gründe:

1. Es handelt sich um ein Ascites Produkt: Wenn Sie dieses Produkt konjugieren, so wie es ist, konjugieren Sie ALLE Proteine, die in dies...

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Thank you for contacting us.

I am afraid the only alternative available against this target previously tested in Mouse tissues is ab684.

This antibody has successfully been tested (and is therefore guaranteed) to target this protein...

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Thank you for your recent telephone enquiry and for your patience.

I can confirm that this particular product is sourced externally. I have been in contact with them in order to obtain this information for you urgently, and regrettably have ...

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Thank you for contacting us.

My source of information for finding the isoform data for this protein was indeed uniprot. See: http://www.uniprot.org/uniprot/P35580

I can also confirm that this antibody is guarenteed to work in mouse....

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Thank you for your enquiry regarding ab684.

This product is not affinity purified but ascitic fluid, thus the concentration is not determined. However, we can provide a range of concentration:.1-10 mg/ml.

You may wish to take a look...

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Thank you for contacting us.

To our knowledge, ab684 has not been tested in immunoprecipitation. However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.
...

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Thank you for your inquiry.

Please confirm to your customer that the immunogen is from the MYH10/Myosin IIB.

I aligned the immunogen with MYH10/Myosin IIA (Q9UKX2) and do not get any homology.

Therefore I can confirm that ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"