Anti-Non Neuronal Enolase antibody [8G8] (ab118866)


  • Product nameAnti-Non Neuronal Enolase antibody [8G8]
    See all Non Neuronal Enolase primary antibodies
  • Description
    Mouse monoclonal [8G8] to Non Neuronal Enolase
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-435 of Human Non Neuronal Enolase with a proprietary tag.

  • Positive control
    • Human breast tissue, Human pancreas tissue, Human heart tissue, HeLa cells, MCF-7 (Human breast adenocarcinoma cell line) lysate and 293T cell transfected lysate.



Our Abpromise guarantee covers the use of ab118866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.


  • FunctionMultifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
  • Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similaritiesBelongs to the enolase family.
  • Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Post-translational
  • Cellular localizationNucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2-phospho-D-glycerate hydro-lyase antibody
    • alpha enolase like 1 antibody
    • Alpha-enolase antibody
    • bh3 interacting domain death agonist antibody
    • c-myc promoter-binding protein 1 antibody
    • C-myc promoter-binding protein antibody
    • c-myc promoter-binding protein-1 antibody
    • eno1 antibody
    • ENO1L1 antibody
    • ENOA_HUMAN antibody
    • enolase 1 (alpha) antibody
    • Enolase 1 antibody
    • HAP 47 antibody
    • HAP47 antibody
    • MBP-1 antibody
    • MPB 1 antibody
    • MPB-1 antibody
    • MPB1 antibody
    • MYC promoter-binding protein 1 antibody
    • NNE antibody
    • Non-neural enolase antibody
    • OTTHUMP00000001706 antibody
    • Phosphopyruvate hydratase antibody
    • Plasminogen-binding protein antibody
    • PPH antibody
    • tau-crystallin antibody
    see all

Anti-Non Neuronal Enolase antibody [8G8] images

  • ab118866 at 5 µg/ml staining Non Neuronal Enolase in Paraffin-embedded Human breast tissue by immunohistochemistry.
  • ab118866 at 5 µg/ml staining Non Neuronal Enolase in Paraffin-embedded Human heart tissue by immunohistochemistry.
  • ab118866 at 5 µg/ml staining Non Neuronal Enolase in Paraffin-embedded Human pancreas tissue by immunohistochemistry.
  • ab118866 at 10 µg/ml staining Non Neuronal Enolase in Paraffin-embedded HeLa tissue by immunofluorescence.
  • All lanes : Anti-Non Neuronal Enolase antibody [8G8] (ab118866)

    Lane 1 : Transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Predicted band size : 47 kDa
  • Anti-Non Neuronal Enolase antibody [8G8] (ab118866) + MCF-7 lysate

    Predicted band size : 47 kDa

References for Anti-Non Neuronal Enolase antibody [8G8] (ab118866)

ab118866 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done. I would like to reassure you that our...

Read More