• Product nameAnti-Non Neuronal Enolase antibody
    See all Non Neuronal Enolase primary antibodies
  • Description
    Rabbit polyclonal to Non Neuronal Enolase
  • SpecificityNNE 100% NSE <0.1% Determined in a competitive ELISA with an antibody dilution of 1/500.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Non Neuronal Enolase (Human)



Our Abpromise guarantee covers the use of ab35075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/50 - 1/250. In a simple ELISA, with 1 µg of antigen coated per well, the 50% OD was observed at an antibody dilution of 1/120. Under the same conditions, the end point was observed at 1/10000.
    IHC-P: 1/25 - 1/100.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 48 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMultifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
      MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
    • Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
    • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
    • Sequence similaritiesBelongs to the enolase family.
    • Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
    • Post-translational
    • Cellular localizationNucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2-phospho-D-glycerate hydro-lyase antibody
      • alpha enolase like 1 antibody
      • Alpha-enolase antibody
      • bh3 interacting domain death agonist antibody
      • c-myc promoter-binding protein 1 antibody
      • C-myc promoter-binding protein antibody
      • c-myc promoter-binding protein-1 antibody
      • eno1 antibody
      • ENO1L1 antibody
      • ENOA_HUMAN antibody
      • enolase 1 (alpha) antibody
      • Enolase 1 antibody
      • HAP 47 antibody
      • HAP47 antibody
      • MBP-1 antibody
      • MPB 1 antibody
      • MPB-1 antibody
      • MPB1 antibody
      • MYC promoter-binding protein 1 antibody
      • NNE antibody
      • Non-neural enolase antibody
      • OTTHUMP00000001706 antibody
      • Phosphopyruvate hydratase antibody
      • Plasminogen-binding protein antibody
      • PPH antibody
      • tau-crystallin antibody
      see all

    References for Anti-Non Neuronal Enolase antibody (ab35075)

    This product has been referenced in:
    • Seliger B  et al. Combined analysis of transcriptome and proteome data as a tool for the identification of candidate biomarkers in renal cell carcinoma. Proteomics 9:1567-81 (2009). Read more (PubMed: 19235166) »
    • Cohen AA  et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB ; Human . Read more (PubMed: 19023046) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (Brain tumor)
    Loading amount 10 µg
    Specification Brain tumor
    Treatment Lysis buffer (urea/thiourea)
    Gel Running Conditions Reduced Denaturing (4-20%)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

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    Submitted Jan 08 2010