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A region within synthetic peptide: GVGLCTGQIK TGAPCRSERL AKYNQLLRIE EELGSKAKFA GRNFRNPLA, corresponding to C terminal amino acids 386-434 of Human Non Neuronal Enolase
Our Abpromise guarantee covers the use of ab49256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:62500.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|