The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 - 8 µg/ml.
Use a concentration of 0.03 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 1 µg/ml.
FunctionMultifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Sequence similaritiesBelongs to the enolase family.
Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
Cellular localizationNucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections) analysis of human kidney tissue labelling Non Neuronal Enolase with ab49343 at 4-8µg/ml. Arrows indicate positively labellled epithelial cells of collecting tubules. Magnification: 400X.
Immunohistochemistry (Frozen sections) - Anti-Non Neuronal Enolase antibody (ab49343)This image is courtesy of an anonymous Abreview
ab49343 staining mouse embryonic brain slice tissue sections by IHC-Fr. Tissue sections were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X in 0.1M PBS. The sample was blocked with 5% serum for 2 hours at 4°C prior to incubation with the primary antibody, diluted 1/20 in 0.1M PBS for 24 hours at 4°C. An Alexa Fluor® 546 conjugated goat anti-rabbit IgG antibody was used as the secondary.
ICC/IF image of ab49343 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49343, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Non Neuronal Enolase antibody (ab49343)
This product has been referenced in:
Sawhney S et al. Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ?133p53a mimic. PLoS One10:e0116270 (2015).
WB, ELISA, IHC-P
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Bentaib A et al. Metabolic reprogramming in transformed mouse cortical astrocytes: A proteomic study. J Proteomics113C:292-314 (2014).
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