Overview

  • Product nameAnti-Non Neuronal Enolase antibody
    See all Non Neuronal Enolase primary antibodies
  • Description
    Mouse monoclonal to Non Neuronal Enolase
  • Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-435 of Human Non Neuronal Enolase

Applications

Our Abpromise guarantee covers the use of ab54979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 47 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionMultifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
  • Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similaritiesBelongs to the enolase family.
  • Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localizationNucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2-phospho-D-glycerate hydro-lyase antibody
    • alpha enolase like 1 antibody
    • Alpha-enolase antibody
    • bh3 interacting domain death agonist antibody
    • c-myc promoter-binding protein 1 antibody
    • C-myc promoter-binding protein antibody
    • c-myc promoter-binding protein-1 antibody
    • eno1 antibody
    • ENO1L1 antibody
    • ENOA_HUMAN antibody
    • enolase 1 (alpha) antibody
    • Enolase 1 antibody
    • HAP 47 antibody
    • HAP47 antibody
    • MBP-1 antibody
    • MPB 1 antibody
    • MPB-1 antibody
    • MPB1 antibody
    • MYC promoter-binding protein 1 antibody
    • NNE antibody
    • Non-neural enolase antibody
    • OTTHUMP00000001706 antibody
    • Phosphopyruvate hydratase antibody
    • Plasminogen-binding protein antibody
    • PPH antibody
    • tau-crystallin antibody
    see all

Anti-Non Neuronal Enolase antibody images

  • Non Neuronal Enolase antibody (ab54979) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human lymphoma tissue.


  • Predicted band size : 47 kDa
  • ICC/IF image of ab54979 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54979, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab54979 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54979, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Non Neuronal Enolase antibody (ab54979)

This product has been referenced in:
  • Cho S  et al. Streptococcus sanguinis and the sera of patients with Behçet's disease stimulate membrane expression of a-enolase in human dermal microvascular endothelial cells. Arch Dermatol Res 305:223-32 (2013). Read more (PubMed: 23131860) »
  • Soh MA  et al. Arsenic, cadmium and neuron specific enolase (ENO2, ?-enolase) expression in breast cancer. Cancer Cell Int 11:41 (2011). IHC-P ; Human . Read more (PubMed: 22098917) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Huh 7.5 cells)
Loading amount 40 µg
Specification Huh 7.5 cells
Gel Running Conditions Reduced Denaturing (12.5% gel)
Blocking step (agent) for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 25 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Cartilage)
Loading amount 25 µg
Specification Cartilage
Gel Running Conditions Reduced Denaturing (4-20% Gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. john wardale

Verified customer

Submitted Sep 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"