Anti-Non Neuronal Enolase antibody (ab87925)

Overview

  • Product name
    Anti-Non Neuronal Enolase antibody
    See all Non Neuronal Enolase primary antibodies
  • Description
    Rabbit polyclonal to Non Neuronal Enolase
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Pig, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Non Neuronal Enolase.

    (Peptide available as ab98944.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; MCF7.

Properties

Applications

Our Abpromise guarantee covers the use of ab87925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 47 kDa).

Target

  • Function
    Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
  • Tissue specificity
    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similarities
    Belongs to the enolase family.
  • Developmental stage
    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localization
    Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2-phospho-D-glycerate hydro-lyase antibody
    • alpha enolase like 1 antibody
    • Alpha-enolase antibody
    • bh3 interacting domain death agonist antibody
    • c-myc promoter-binding protein 1 antibody
    • C-myc promoter-binding protein antibody
    • c-myc promoter-binding protein-1 antibody
    • eno1 antibody
    • ENO1L1 antibody
    • ENOA_HUMAN antibody
    • enolase 1 (alpha) antibody
    • Enolase 1 antibody
    • HAP 47 antibody
    • HAP47 antibody
    • MBP-1 antibody
    • MPB 1 antibody
    • MPB-1 antibody
    • MPB1 antibody
    • MYC promoter-binding protein 1 antibody
    • NNE antibody
    • Non-neural enolase antibody
    • OTTHUMP00000001706 antibody
    • Phosphopyruvate hydratase antibody
    • Plasminogen-binding protein antibody
    • PPH antibody
    • tau-crystallin antibody
    see all

Images

  • All lanes : Anti-Non Neuronal Enolase antibody (ab87925) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds Human Alpha-enolase contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ICC/IF image of ab87925 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87925, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgX (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in % PFA fixed (10 min) HeLa cells at 1µg/ml.

References

ab87925 has not yet been referenced specifically in any publications.

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