Anti-Nonspecific Cytotoxic Cells antibody [5C.6] (ab2778)

Overview

  • Product name
    Anti-Nonspecific Cytotoxic Cells antibody [5C.6]
  • Description
    Mouse monoclonal [5C.6] to Nonspecific Cytotoxic Cells
  • Tested applications
    Suitable for: ELISA, WB, Flow Cyt, Inhibition Assay, IPmore details
  • Species reactivity
    Reacts with: Rat, Horse, Cow, Dog, Pig, Tilapia, Catfish
  • Immunogen

    Other Immunogen Type corresponding to Nonspecific Cytotoxic Cells. Purified NCC cells from catfish.

  • Positive control
    • This antibody as been successfully used in cytotoxic inhibition studies with NCC/NK/LAK cells, panning, and ELISA procedures. In cytotoxicity assays, this antibody is used under saturating conditions to inhibit target cell conjugation.
  • General notes

    U.S. Patent Numbers: 5,028,424 and 5,229,494.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • Purity
    Purified IgM
  • Primary antibody notes
    Non-specific cytotoxic cells (NCCs) in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. One such property is that NCC/natural killer (NK)/lymphokine activated killer (LAK) cells express spontaneous, non-major histocompatibility complex restricted cytotoxic activity. NCC and LAK lyse a variety of transformed murine and human B-cell, T-cell and myeloid targets. A 32 kDa membrane protein [non-specific cytotoxic cell receptor protein (NCCRP-1)] expressed by NCC and certain mammalian NK/LAK cells mediates this cytotoxicity. NCCRP-1 is evolutionarily conserved and is found in species ranging from marine and freshwater teleosts to higher mammals.
  • Clonality
    Monoclonal
  • Clone number
    5C.6
  • Isotype
    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 10 µg/ml.

By Western blot, this antibody detects a 32 kDa protein representing nonspecific cytotoxic cell receptor protein.

Flow Cyt Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

References

This product has been referenced in:
  • Jaso-Friedmann L  et al. NCCRP-1: a novel receptor protein sequenced from teleost nonspecific cytotoxic cells. Mol Immunol 34:955-65 (1997). Read more (PubMed: 9464530) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us. I received the following information from the lab: We don’t have the specific protocol used to test this antibody in flow cytometry. This product was developed outside our lab, and upon checking our files, I ...

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I have been informed by the suppliers for ab37131 and ab2778 that we do not supply the standard proteins unfortunately. For ab2778, anti-Nonspecific Cytotoxic Cell antibody, the lab team has informed me that this antibody was actually tested in ELIS...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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